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Related Experiment Videos

Surface components affecting interactions between Neisserai gonorrhoeae and eucaryotic cells.

J Swanson

    The Journal of Infectious Diseases
    |August 1, 1977
    PubMed
    Summary

    Pili on Neisseria gonorrhoeae enhance attachment to non-immune cells but reduce interaction with immune cells like macrophages. Bacterial adherence variations correlate with specific proteins and trypsin susceptibility.

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    Area of Science:

    • Microbiology
    • Immunology
    • Cell Biology

    Background:

    • Neisseria gonorrhoeae interactions with host cells are crucial for infection.
    • Bacterial pili are known virulence factors influencing host cell adhesion.
    • The role of pili in interacting with both non-immune and immune cells requires further clarification.

    Purpose of the Study:

    • To investigate the role of pili in Neisseria gonorrhoeae interactions with various eukaryotic cells.
    • To determine how pili affect bacterial association with non-leukocytic cells versus phagocytic immune cells.
    • To explore the relationship between bacterial adherence, protein profiles, and susceptibility to host defenses.

    Main Methods:

    • Studying Neisseria gonorrhoeae interactions with tissue culture cells, human sperm, and buccal mucosal cells.

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  • Assessing the influence of pili on bacterial attachment and association.
  • Examining the effect of pili on interactions with mouse peritoneal macrophages and neutrophils.
  • Analyzing bacterial adherence variations, colony forms, protein patterns via polyacrylamide electrophoresis, and trypsin susceptibility.

    • Main Results:

    • Piliation of Neisseria gonorrhoeae enhances attachment to non-leukocytic eukaryotic cells.
    • Pili reduce the interaction of Neisseria gonorrhoeae with mouse peritoneal macrophages.
    • Pili decrease gonococcal phagocytosis by neutrophils, a finding secondary to a 'leukocyte-association' factor.
    • Bacterial adherence differences correlate with colony morphology, protein patterns, and trypsin susceptibility.

    Conclusions:

    • Pili play a dual role in Neisseria gonorrhoeae pathogenesis, facilitating host cell colonization while evading certain immune responses.
    • A 'leukocyte-association' factor, linked to specific bacterial proteins, influences gonococcal interactions with immune cells.
    • Variations in Neisseria gonorrhoeae adherence and protein expression contribute to differential interactions and susceptibility to host defenses.