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Related Experiment Video

Updated: Sep 10, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

Computational Tools and Resources for Long-read Metagenomic Sequencing Using Nanopore and PacBio.

Tianyuan Zhang^1,2, Mian Jiang², Hanzhou Li²

¹Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.

Genomics, Proteomics & Bioinformatics

|August 22, 2025

Summary

Advancements in long-read sequencing technologies like Nanopore and PacBio are revolutionizing shotgun metagenomics. This review details their impact on analyzing microbial communities and provides essential computational resources for researchers.

Keywords:

Database Metagenome Nanopore PacBio Software

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Last Updated: Sep 10, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

Area of Science:

Microbiology
Genomics
Bioinformatics

Background:

Shotgun metagenomics analyzes microbial communities directly from environmental samples.
Next-generation sequencing, especially long-read technologies, has significantly advanced the field.
These technologies overcome limitations of previous methods, enabling deeper analysis of microbial composition and function.

Purpose of the Study:

To provide a comprehensive review of long-read metagenomics.
To highlight historical developments and significant advancements.
To explore diverse applications and essential computational resources.

Main Methods:

Review of historical development of long-read metagenomics.
Exploration of applications across various scientific fields.
Summary of computational resources (software, databases, packages).

Main Results:

Long-read sequencing platforms (Nanopore, PacBio) enhance metagenomic data assembly and analysis.
Improved ability to analyze microbial communities without cultivation.
Identification of key computational tools and resources for efficient analysis.

Conclusions:

Long-read metagenomics offers unprecedented depth in exploring microbial life.
The review provides a roadmap for resource utilization and innovation in the field.
Practical guidance on software installation and use is offered via GitHub.