Pyrococcus furiosus Argonaute biosensing platform based on Hemin@ZIF-90 nanozyme-modulated signal amplification for...

Foodborne pathogens have posed a great threat to food safety and public health, and thus the development of accurate and sensitive methods for the detection of foodborne pathogens is critical. In this study, a novel Pyrococcus furiosus Argonaute (PfAgo) biosensing platform based on metal-organic framework (MOF) nanozyme (namely AgoZyme) was developed for the detection of Salmonella typhimurium. Inspired by the catalytic characteristics of horseradish peroxidase, we synthesized a Hemin@ZIF-90 nanozyme with peroxidase-like activity by encapsulating hemin within a zeolitic imidazolate framework-90 (ZIF-90) through a self-assembly strategy. By integrating the specific recognition and cleavage capabilities of PfAgo with the high catalytic performance of Hemin@ZIF-90, the AgoZyme biosensor exhibited excellent detection sensitivity and specificity. This biosensor had a limit of detection of 1 CFU/mL in a linear range from 10¹ to 10⁸ CFU/mL. This work provided a new insight for designing and developing novel biosensors for food safety detection.