The power of two: Combining imaging and sequencing as readouts for in vivo pooled genetic screens

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Carlos Jiménez1, Nicola Crosetto2, Magda Bienko2

  • 1Human Technopole, Milan, Italy.

Molecular Cell
|August 22, 2025
PubMed

Abstract:

In a recent publication in Cell, Saunders et al.1 describe an important step forward for in vivo genetic screens, by enabling parallel tissue imaging and sequencing, boosting the discovery of gene-phenotype links that had previously gone unnoticed.

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Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

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