Multidimensional strategies for efficient heterologous protein expression in Aspergillus niger

Area of Science:

• Biotechnology
• Microbiology
• Synthetic Biology

Background:

• Microbial protein is a sustainable alternative to animal and plant proteins.
• Aspergillus niger, a GRAS microorganism, is widely used for producing heterologous proteins.
• Current limitations in Aspergillus niger include gene transcription, metabolic flux, protein folding, and secretion.

Purpose of the Study:

• To review recent advancements in enhancing heterologous protein production using Aspergillus niger.
• To address the challenges in constructing universal Aspergillus niger chassis cells.
• To provide insights into efficient and sustainable protein production.

Main Methods:

• Exploration of advanced molecular biology tools and metabolic engineering strategies.
• Review of progress in expression systems, secretion pathways, and metabolic flux optimization.
• Discussion of intelligent fermentation and multi-omics integration for systematic optimization.

Main Results:

• Synthetic biology tools have significantly improved protein production efficiency.
• Multi-dimensional strategies enhance gene transcription, protein folding, and secretion.
• Intelligent fermentation and multi-omics integration offer systematic optimization approaches.

Conclusions:

• Aspergillus niger holds great potential for efficient and sustainable heterologous protein production.
• Continued advancements in synthetic biology and metabolic engineering are crucial.
• Optimized Aspergillus niger chassis cells can revolutionize industrial protein manufacturing.