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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Related Experiment Video

Updated: Sep 10, 2025

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

Published on: May 28, 2021

Time-deterministic cryo-optical microscopy.

Kosuke Tsuji^1,2, Masahito Yamanaka³, Yasuaki Kumamoto^1,4

¹Department of Applied Physics, Graduate School of Engineering, The University of Osaka, Osaka, Japan.

Light, Science & Applications

|August 22, 2025

Summary

This summary is machine-generated.

Researchers developed rapid freezing microscopy to capture fast cellular dynamics. This technique improves imaging quality and preserves cellular states for detailed biological insights.

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Last Updated: Sep 10, 2025

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Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

Area of Science:

Cell Biology
Microscopy Techniques
Biophysics

Background:

Fluorescence microscopy visualizes cellular processes but struggles with high signal-to-noise ratio (SNR) at fast acquisition rates.
Observing rapid cellular dynamics requires high SNR imaging, which is a significant challenge in current microscopy.
Existing fixation methods can alter cellular morphology and conditions, limiting dynamic studies.

Purpose of the Study:

To develop a rapid freezing technique for capturing cellular dynamics during optical microscopy.
To combine the benefits of live-cell imaging (dynamics) and cryofixation (high SNR).
To preserve cellular morphology, molecular, and ionic states at specific time points.

Main Methods:

Developed a millisecond-scale rapid freezing method integrated with optical microscopy.
Applied the technique to fluorescence and Raman microscopy under low-temperature conditions.
Utilized fluorescent ion indicators to visualize intracellular calcium dynamics.

Main Results:

Achieved high spatial resolution and quantification with improved SNR snapshots.
Successfully preserved cellular morphology and conditions compared to chemical fixation.
Demonstrated time-deterministic suspension and visualization of intracellular calcium dynamics.
Confirmed spatial and temporal fixation of ion distribution and probe molecule conformation.

Conclusions:

The rapid freezing technique effectively captures cellular dynamics with high fidelity.
This method offers a powerful approach for detailed insights into biological processes with enhanced spatial and temporal accuracy.
Combines live-cell and cryofixation microscopy advantages for dynamic biological imaging.