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Oncogenes and bladder cancer.

P R Malone, K V Visvanathan, B A Ponder

British Journal of Urology

|December 1, 1985

Summary

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Researchers identified an activated c-H-ras-1 oncogene in one bladder cancer patient using a NIH/3T3 transfection assay. Gene amplification or rearrangement was not detected in any tumors, leaving the role of ras oncogenes in bladder cancer pathogenesis unclear.

Area of Science:

Oncology
Molecular Biology
Genetics

Background:

Transitional cell carcinoma is the most common type of bladder cancer.
Oncogenes, such as ras, are known to play a role in cancer development.
The specific role of ras oncogene activation in human bladder carcinoma pathogenesis requires further investigation.

Purpose of the Study:

To screen bladder tumors for transforming activity using the NIH/3T3 transfection assay.
To investigate the potential involvement of the c-H-ras-1 gene in bladder cancer through gene amplification or rearrangement.
To determine if activated oncogenes are present in transitional cell carcinomas of the bladder.

Main Methods:

Screening of 15 superficial and invasive transitional cell carcinomas of the bladder.

Related Experiment Videos

NIH/3T3 transfection assay to detect transforming activity.

Southern blot analysis of tumor and normal lymphocyte DNA to assess c-H-ras-1 gene amplification or rearrangement.

Main Results:

One patient with a grade 2 T1 tumor exhibited an activated oncogene via the NIH/3T3 transfection assay.
The activated oncogene was identified as a c-H-ras-1 gene.
No evidence of c-H-ras-1 gene amplification or rearrangement was found in any of the analyzed tumors.

Conclusions:

An activated c-H-ras-1 oncogene was detected in a subset of human bladder transitional cell carcinomas.
The study did not find evidence of c-H-ras-1 gene amplification or rearrangement in the screened tumors.
The precise significance of ras oncogene activation in the pathogenesis of human bladder carcinoma remains undetermined.