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Related Concept Videos

iChip01:24

iChip

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The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...
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Automated Microbial Diagnostics01:24

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Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
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Related Experiment Video

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Microfluidic Chip Fabrication and Method to Detect Influenza
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Intraoperative pathogen rapid detection on chip.

Quanying Fu1, Zhikai Wu2, Shaoqiong Feng1

  • 1Guangdong Provincial Key Laboratory of Sensing Technology and Biomedical Instrument, School of Biomedical Engineering, Shenzhen Campus of Sun Yat-sen University, Shenzhen, 518107, China.

Biosensors & Bioelectronics
|August 23, 2025
PubMed
Summary
This summary is machine-generated.

A new intraoperative pathogen rapid detection (IPRD) method identifies live pathogens in surgical fluid within 15 minutes. This rapid diagnostic tool aids surgeons in timely treatment decisions for surgical site infections.

Keywords:
Intraoperative pathogen rapid detectionNucleic acid detectionOn-chip technologyPercutaneous nephrolithotomyViable fraction detection

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Area of Science:

  • Medical Diagnostics
  • Microbiology
  • Surgical Innovation

Background:

  • Surgical site infections are a significant risk, potentially leading to sepsis.
  • Accurate and rapid pathogen identification intraoperatively is crucial for effective treatment.
  • Current methods for pathogen detection are often too slow or cannot distinguish live from dead organisms.

Purpose of the Study:

  • To develop a rapid, chip-based method for intraoperative pathogen detection.
  • To simultaneously identify pathogen presence, viability, species, and concentration.
  • To provide timely information for surgical and antibiotic therapy decisions.

Main Methods:

  • A novel intraoperative pathogen rapid detection (IPRD) method was developed.
  • The method utilizes a chip with two parts: live/dead staining with AI microscopy and electroporation-based lysis followed by LAMP assay.
  • Simultaneous detection of multiple pathogens, viability, and concentration in 15 minutes.

Main Results:

  • The IPRD method achieved 99.01% accuracy in clinical validation.
  • It reliably detected Candida albicans, Escherichia coli, and Enterococcus faecalis at 10^3 CFU/mL.
  • The assay provides comprehensive pathogenic information within a clinically relevant timeframe.

Conclusions:

  • The IPRD method offers a significant advancement in intraoperative diagnostics.
  • It enables rapid and accurate identification of live pathogens in surgical fluid.
  • This technology has potential applications in infection-prone surgeries, improving patient outcomes.