Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

17.4K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.4K
DNA Microarrays02:34

DNA Microarrays

18.4K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
18.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Moderators of treatment response in late-life depression.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology·2026
Same author

Memoriam for James A. Greene, MD, DLFAPA, FACPsych.

The American journal of geriatric psychiatry : official journal of the American Association for Geriatric Psychiatry·2026
Same author

Association of Subjective Social Support With Antidepressant Treatment Response Among Older Depressed Adults in the Neurobiology of Late-Life Depression Study.

The American journal of geriatric psychiatry. Open science, education, and practice·2026
Same author

Right anterior supramarginal gyrus-cerebellum hyperconnectivity and rumination partially mediate the link between childhood abuse and anger in late-life depression.

Journal of affective disorders·2025
Same author

The Independent Effect of Depression on Financial Decision-Making in Older Adults.

The American journal of geriatric psychiatry : official journal of the American Association for Geriatric Psychiatry·2025
Same author

Iterative learning for joint image denoising and motion artifact correction of 3D brain MRI.

Medical image analysis·2025
Same journal

AI-driven photophysics-aware design of fluorescent probes with applications in α-synuclein biosensing and inhibitor screening.

Biosensors & bioelectronics·2026
Same journal

Three-dimensional helical integration of high-density linear microelectrode arrays and their cross-tissue applications.

Biosensors & bioelectronics·2026
Same journal

Integration of electrochemical sensors in organ-on-a-chip microfluidic platforms: Advances and perspectives.

Biosensors & bioelectronics·2026
Same journal

DNN-PURE: A deep neural network approach to paper-based urea sensing.

Biosensors & bioelectronics·2026
Same journal

Rationally architected MOF-derived Co<sub>3</sub>O<sub>4</sub>@NiMn-LDH hollow heterostructure-based sensor array empowering sensitive detection and discrimination of neurological biomarkers.

Biosensors & bioelectronics·2026
Same journal

Four-in-one multifunctional CoCu-NC@AuPt nanozyme integrated M13 phage-displayed nanobody based multimodal lateral flow immunoassay for bovine lactoferrin detection.

Biosensors & bioelectronics·2026
See all related articles

Related Experiment Video

Updated: Sep 10, 2025

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.5K

ECL-CRISPR array for multiplexed detection of miRNAs.

Keshani Hiniduma1, P I Thilini De Silva1, Rachelle Canete1

  • 1Department of Chemistry and Institute of Materials Science, University of Connecticut, Storrs, CT, 06269-3060, USA.

Biosensors & Bioelectronics
|August 23, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a CRISPR/Cas13a-based electrochemiluminescent (ECL) array for detecting Alzheimer's disease (ALZ) microRNAs. The novel biosensor offers highly sensitive and specific detection of key miRNA biomarkers.

More Related Videos

Performing Custom MicroRNA Microarray Experiments
07:04

Performing Custom MicroRNA Microarray Experiments

Published on: October 28, 2011

19.6K
Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR qPCR Arrays
10:58

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR qPCR Arrays

Published on: December 3, 2010

17.4K

Related Experiment Videos

Last Updated: Sep 10, 2025

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.5K
Performing Custom MicroRNA Microarray Experiments
07:04

Performing Custom MicroRNA Microarray Experiments

Published on: October 28, 2011

19.6K
Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR qPCR Arrays
10:58

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR qPCR Arrays

Published on: December 3, 2010

17.4K

Area of Science:

  • Biomedical Engineering
  • Molecular Biology
  • Analytical Chemistry

Background:

  • MicroRNAs (miRNAs) are crucial biomarkers for Alzheimer's disease (AD).
  • Existing detection methods for miRNAs often lack sensitivity, specificity, or require complex instrumentation.
  • CRISPR/Cas13a systems offer programmable RNA targeting capabilities.

Purpose of the Study:

  • To develop a novel electrochemiluminescent (ECL) array for sensitive and specific detection of Alzheimer's disease (AD)-related miRNAs.
  • To utilize the CRISPR/Cas13a system for miRNA recognition and signal amplification in an ECL platform.
  • To validate the performance of the developed array for detecting specific AD miRNA biomarkers in biological samples.

Main Methods:

  • Development of a 3D-printed ECL array integrated with CRISPR/Cas13a.
  • Target miRNA binding to Cas13a activates its RNase activity, cleaving poly-r-guanosine (poly-r-G).
  • Cleavage of poly-r-G generates ECL activators that enhance signal output from a ruthenium-based film (RuPVP).

Main Results:

  • The ECL array successfully detected three Alzheimer's disease (AD) miRNA biomarkers (miR30e-5p, miR34c-3p, miR200c-5p).
  • Achieved ultra-low limits of detection (LOD) ranging from 0.074 fg/mL to 42 pg/mL.
  • Demonstrated high sensitivity, wide linear dynamic ranges (70 pg/mL to 70 μg/mL), and excellent correlation with a fluorescence-based CRISPR method.

Conclusions:

  • The CRISPR/Cas13a-based ECL array provides a highly sensitive and specific platform for detecting AD miRNA biomarkers.
  • This innovative approach holds promise for early diagnosis and monitoring of Alzheimer's disease.
  • The 3D-printed array design offers potential for multiplexed biomarker detection in clinical diagnostics.