Point-of-care devices for thin-film-based biosensors for the detection of glucose, urea, and pH in biological samples
Sandeep Choudhary1, Tanmay Vyas2, Abhijeet Joshi2
1Mehta Family School of Biosciences and Biomedical Engineering, Indian Institute of Technology Indore, Simrol, Khandwa Road, Indore 453552, Madhya Pradesh, India; Department of Biomedical Engineering, School of Engineering and Technology, Central University of Rajasthan, NH -8 Bandarsindri, Ajmer 305817 Rajasthan, India.
Abstract:
The current work focuses on the development and testing of point-of-care (POC) devices-based on a color sensor (CSD) and a fiber-optic spectrometer (FOS)-for healthcare monitoring of biological markers. Agarose-based fluorescent indicator-loaded thin film biosensors (TFBs) were synthesized by co-immobilizing analyte-specific enzymes such as glucose oxidase and urease. This study further evaluates enzyme-immobilized polymer-fluorophore-based thin films for the detection of glucose, urea, and pH in plasma and urine samples. TFB-CSD and TFB-FOS biosensor-coupled devices demonstrated linear detection ranges for glucose (0-40 mM), urea (0-100 mM), and pH (4-9), with rapid response times of 30 s for pH sensing and 2 min for glucose and urea sensing. The sequential results obtained using TFB-CSD were compared with those from TFB-FOS. Both devices were found to accurately detect analytes (expressed as average % recovery): glucose (100.32 % for TFB-FOS and 103.59 % for TFB-CSD), urea (114.30 % for TFB-FOS and 108.49 % for TFB-CSD), and pH (103.49 % for TFB-FOS and 105.21 % for TFB-CSD) in blood and urine samples.
