Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

13.7K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
13.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[ATM/H2AX and repair of sperm-DNA damage during cryopreservation].

Zhonghua nan ke xue = National journal of andrology·2011
Same author

Predicting accident frequency at their severity levels and its application in site ranking using a two-stage mixed multivariate model.

Accident; analysis and prevention·2011
Same author

Photothermally enhanced photodynamic therapy delivered by nano-graphene oxide.

ACS nano·2011
Same author

[Characteristics of soil respiration in Phyllostachys edulis forest in Wanmulin Natural Reserve and related affecting factors].

Ying yong sheng tai xue bao = The journal of applied ecology·2011
Same author

Quality changes in sea urchin (Strongylocentrotus nudus) during storage in artificial seawater saturated with oxygen, nitrogen and air.

Journal of the science of food and agriculture·2011
Same author

Global effect of an RNA polymerase β-subunit mutation on gene expression in the radiation-resistant bacterium Deinococcus radiodurans.

Science China. Life sciences·2011
Same journal

Micro- and nanoplastics as emerging clinical analytes: analytical validation, interpretive uncertainty, and laboratory actionability in human specimens.

Clinica chimica acta; international journal of clinical chemistry·2026
Same journal

Performance of Free Light Chain reagents in the Dutch External Quality Assessment programme over the past 14 years.

Clinica chimica acta; international journal of clinical chemistry·2026
Same journal

CRISPR-Cas12a-based liquid biopsy technology: challenges in large-scale clinical application.

Clinica chimica acta; international journal of clinical chemistry·2026
Same journal

Diagnostic biomarkers, prognostic signatures, and therapeutic resistance: shedding light on the dual role of autophagy in glioblastoma.

Clinica chimica acta; international journal of clinical chemistry·2026
Same journal

Artificial intelligence in diagnostic software: validation, safety, and lifecycle challenges in Europe.

Clinica chimica acta; international journal of clinical chemistry·2026
Same journal

Strategies for effective occurrence Management in Resource-Limited Clinical Laboratories: Challenges and practical solutions.

Clinica chimica acta; international journal of clinical chemistry·2026
See all related articles

Related Experiment Video

Updated: Sep 10, 2025

Quality-Controlled Sputum Analysis by Flow Cytometry
07:22

Quality-Controlled Sputum Analysis by Flow Cytometry

Published on: August 9, 2021

5.3K

Technologic advances in flow cytometry.

Xinxin Liu1, Chao Wang1, Xiuru Guan1

  • 1The First Affiliated Hospital of Harbin Medical University, Department of Laboratory Diagnostics, Harbin 150001, China.

Clinica Chimica Acta; International Journal of Clinical Chemistry
|August 23, 2025
PubMed
Summary
This summary is machine-generated.

Flow cytometry has advanced significantly over 60 years, enhancing single-cell analysis sensitivity and enabling new clinical applications. Further research is needed to address challenges in data processing and technology translation.

Keywords:
Flow cytometryImaging flow cytometryMass cytometryNanoparticle flow cytometrySpectral flow cytometry

More Related Videos

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

10.4K
Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry
11:08

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Published on: May 5, 2017

13.1K

Related Experiment Videos

Last Updated: Sep 10, 2025

Quality-Controlled Sputum Analysis by Flow Cytometry
07:22

Quality-Controlled Sputum Analysis by Flow Cytometry

Published on: August 9, 2021

5.3K
Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

10.4K
Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry
11:08

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Published on: May 5, 2017

13.1K

Area of Science:

  • Biotechnology
  • Cell Biology
  • Medical Diagnostics

Background:

  • Flow cytometry is a pivotal single-cell analysis technique with a 60-year developmental history.
  • Technological evolution has transformed it from basic fluorescence labeling to advanced multi-modal analysis.

Purpose of the Study:

  • To systematically review the technological progression of flow cytometry.
  • To highlight its impact on precision medicine and interdisciplinary research.
  • To provide a reference framework for future advancements.

Main Methods:

  • Review of conventional fluorescence, spectral, mass, imaging, and nanoparticle flow cytometry.
  • Analysis of technological advancements and their biological implications.
  • Examination of clinical applications and remaining challenges.

Main Results:

  • Significant enhancements in sensitivity and dimensionality of single-cell analysis.
  • Facilitation of clinical applications such as immunotherapy monitoring, cardiovascular risk assessment, and infectious disease diagnosis.
  • Identification of ongoing challenges in high-dimensional data processing and standardization.

Conclusions:

  • Flow cytometry's evolution has greatly expanded biological understanding and clinical utility.
  • Continued innovation is crucial for overcoming current limitations and advancing precision medicine.
  • The review serves as a foundational reference for interdisciplinary flow cytometry research.