Establishment of a mouse melanocyte clone which synthesizes both eumelanin and pheomelanin

We isolated cultured melanocyte population from dorsal skin of C57BL/6J (genotype, a/a; C/C) new-born mouse and established several cell lines. One of the melanocyte clones, TM 10, produces both eumelanin and pheomelanin. This cell line seems to be suitable for study of the regulation mechanism of production of these pigments in vitro.