Related Experiment Video
Updated: Sep 10, 2025

Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts
Published on: July 25, 2020
[Corrigendum] Identification of Akt1 as a potent therapeutic target for oral squamous cell carcinoma
Koh-Ichi Nakashiro1, Hiroshi Tanaka1, Hiroyuki Goda1
1Department of Oral and Maxillofacial Surgery, Ehime University Graduate School of Medicine, Toon, Ehime 791-0295, Japan.
Abstract:
Following the publication of the above paper, it was drawn to the Editor's attention by a concerned reader that, regarding the western blots shown in Figs. 1C and 3A, the six lanes for the control β‑tubulin blots shown in Fig. 1C appeared strikingly similar to the β‑tubulin blots in Fig. 3A, albeit the bands in Fig. 3A appeared to have been horizontally stretched. The authors were able to check their original data, and realized that Fig. 3 had inadvertently been assembled incorrectly. A revised version of Fig. 3, now showing data for both the Akt1 and β‑tubulin blots from one of the repeated experiments, is shown opposite. All authors confirm that the errors made in assembling Fig. 3 did not have a major impact on the conclusions reported in the above article, and they thank the Editor of International Journal of Oncology for allowing them the opportunity to publish a Corrigendum. Furthermore, all the authors agree to the publication of this Corrigendum, and apologize to the readers for any inconvenience caused. [International Journal of Oncology 47: 1273‑1281, 2015; DOI: 10.3892/ijo.2015.3134].
Insights
A scientific corrigendum clarifies an error in figure assembly for western blots in a published study. The authors confirm the error did not significantly impact the study's conclusions.
Area of Science:
- Biochemistry
- Molecular Biology
- Oncology Research
Context:
- A previously published study in International Journal of Oncology faced scrutiny regarding western blot figures.
- A reader identified potential similarities and discrepancies between control beta-tubulin blots in Fig. 1C and Fig. 3A.
Purpose:
- To address and correct an inadvertent error in the assembly of Fig. 3.
- To provide a revised figure (Fig. 3) with accurate representation of Akt1 and beta-tubulin blots.
- To maintain scientific integrity and transparency.
Summary:
- The authors identified an error where Fig. 3 was assembled incorrectly, leading to a visual similarity between beta-tubulin blots from different figures.
- A corrected Fig. 3 is presented, displaying data from repeated experiments for both Akt1 and beta-tubulin.
- The authors verified that this assembly error did not substantially alter the original study's conclusions.
Impact:
- Ensures accuracy of published scientific data, specifically western blot representations.
- Upholds the credibility of the research findings by correcting procedural errors.
- Provides clarity to readers and the scientific community regarding the western blot data.
More Related Videos
05:45In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System
Published on: January 9, 2020
09:29Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies
Published on: September 30, 2016
Related Concept Videos
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
Targeted Cancer Therapies
There are several types of targeted therapies against...
Abnormal Proliferation
M-Cdk Drives Transition Into Mitosis
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Inhibition of Cdk Activity
PI3K/mTOR/AKT Signaling Pathway