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Engineering cyclized biomolecules for advanced diagnostic and therapeutic applications
Yeonju Lee1, Bohyun Oh1, Sumin Kang1
1Department of Life Science, Hanyang University, Seoul 04763, Republic of Korea.
Biotechnology Advances
|August 24, 2025
Summary
Cyclizing biomolecules like nucleic acids and proteins enhances their stability and biological activity. This engineering approach improves targeted therapies and diagnostics, expanding their use in biotechnology.
Area of Science:
- Biotechnology and Biomolecular Engineering
- Molecular Biology and Biochemistry
Background:
- Nucleic acids, peptides, and proteins are vital biomolecules with diverse biological roles.
- Their therapeutic and diagnostic applications are limited by instability and inefficient delivery.
- Cyclization offers a strategy to overcome these limitations.
Purpose of the Study:
- To review engineering methods for creating cyclized biomolecules.
- To explore the applications of cyclized biomolecules in biosensing, imaging, and delivery.
- To highlight rationally engineered cyclized constructs for expanded theranostic applications.
Main Methods:
- Review of diverse engineering strategies for biomolecule cyclization.
- Analysis of structure-activity relationships in cyclic biomolecules.
- Examination of applications in targeted diagnosis and therapy.
Main Results:
- Cyclization enhances conformational rigidity, stability, and biological activity of biomolecules.
- Cyclic architectures improve antitumor, anti-inflammatory, and anti-infective properties.
- Precise control over binding affinity, stability, and membrane permeability is achieved through cyclization.
Conclusions:
- Cyclized biomolecules offer enhanced theranostic potential beyond naturally occurring forms.
- Engineering cyclized constructs expands their utility in biotechnology and medicine.
- Cyclization is a key strategy for developing advanced diagnostic and therapeutic agents.
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