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Updated: Sep 10, 2025

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Step-feeding reactor establishment for advanced nitrogen removal from mature landfill leachate via double anammox
Shixin Jing1, Qiong Zhang1, Zhaozhi Wang1
1National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
Abstract:
To address the engineering challenges of high costs, NO2- sensitivity, and carrier dependency are associated with traditional nitrogen removal processes for treating mature landfill leachate (MLL), this study developed an innovative step-feeding double anammox process. By establishing a pure floc sludge system with gradient carbon source allocation strategy, the nitrogen removal mechanisms under several influent distribution ratios (1:1:1, 4:4:2, 5:3:2) were systematically investigated. The system achieved an anammox contribution of 91.4 ± 0.2 % and a total nitrogen removal efficiency of 96.4 ± 0.2 % with an effluent total inorganic nitrogen concentration of 14.9 ± 0.4 mg/L under the optimal influent distribution ratio of 4:4:2. Microbial community analysis revealed that the relative abundance of Candidatus_Brocadia reached 2.91 % in pure floc sludge, confirming successful anammox bacteria enrichment without carriers and granular sludge. The process effectively balanced functional microbial activity and operational costs, providing an energy-efficient way for advanced nitrogen removal from MLL.
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