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Related Experiment Video

Updated: Sep 10, 2025

Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair

Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair

Published on: February 17, 2021

High-Resolution Spatial Transcriptomics Unveils Spatially Resolved Gene Modules and Fatty Acid Metabolism

Yin Li¹, Wenwan Zhang¹, Yao Teng²

¹Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.

The Journal of Investigative Dermatology

|August 24, 2025

Summary

Human skin aging involves cellular changes, with reduced fatty acid synthesis contributing to senescence and barrier dysfunction. This study reveals new molecular mechanisms for skin aging interventions.

Keywords:

Human skin Skin aging Spatial transcriptomics Stereo-seq

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Last Updated: Sep 10, 2025

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Published on: March 14, 2022

Area of Science:

Dermatology
Genomics
Molecular Biology

Background:

Human skin aging involves complex cellular and molecular changes.
High-resolution spatial transcriptomics is key to understanding these age-related alterations.

Purpose of the Study:

To create a high-resolution spatial transcriptomic atlas of human eyelid skin.
To identify molecular mechanisms driving skin aging and potential intervention targets.

Main Methods:

Stereo-seq for spatial transcriptomic analysis.
Multiplexed fluorescent in situ hybridization (FISH) for spatial mapping.
Functional studies using keratinocytes and a T-Skin model.

Main Results:

Identified 8 spatial tissue clusters and 18 gene modules in aging skin.
Revealed decreased dermal-epidermal communication, reduced fatty acid synthesis, and epidermal cell identity loss.
Showed reduced FDPS and FASN activity promotes epidermal senescence and barrier dysfunction.

Conclusions:

Spatially resolved transcriptomics offers novel insights into skin aging mechanisms.
Reduced fatty acid synthesis is a key driver of age-related skin deterioration.
Findings suggest new targets for anti-aging interventions.