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Updated: Jun 24, 2026
Microwave Assisted Rapid Diagnosis of Plant Virus Diseases by Transmission Electron Microscopy
Published on: October 14, 2011
Field-parallel six-sample microfluidic detection of plant viruses via raffinose-assisted one-pot LAMP-CRISPR/Cas12b
Jiahui Wang1, Wenqing Yang1, Jingjing Fang1
1College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, China.
Introduction:
Plant viral diseases threaten food security globally. Rapid, accurate and early detection of viral pathogens is critical for proactive surveillance and effective control.
Objectives:
This study aimed to develop a platform for the diagnosis of plant viruses in the field.
Methods:
We developed an ALERT platform (AapCas12b coupled RT-LAMP Extraction-field RNA testing) that integrates RT-LAMP and CRISPR/Cas12b-based lateral flow system for the diagnosis of plant viruses. The portable metal incubator, coupled with the integrated microfluidic chip, enables parallel detection of six test samples in the field. Additionally, a raffinose additive solution effectively achieved the phase separation between the LAMP and CRISPR/Cas12b system through promoting dynamic diffusion of LAMP products amplicons.
Results:
The ALERT platform exhibited comparable sensitivity to conventional RT-PCR, and field detection showed 100% diagnostic agreement with RT-PCR.
Conclusions:
This platform exhibits outstanding performance with high sensitivity and portability, and has great potential for rapid plant virus detection, especially in environments with resource-limited settings.
