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Injectability of temperature-responsive hydrogel derived from elastin-like polypeptide for cell delivery.

Mutawakil Al Muqadasi1, Keitaro Ii1, Kei Nishida2

  • 1Department of Life Science and Technology, School of Life Science and Technology, Institute of Science Tokyo, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.

Journal of Bioscience and Bioengineering
|August 24, 2025
PubMed
Summary
This summary is machine-generated.

This study shows coiled-coil unit-bound elastin-like polypeptides (CUBEs) form injectable hydrogels for cell delivery. The O-CUBE system successfully gels in situ and supports cell survival and proliferation, demonstrating its potential for minimally invasive tissue engineering.

Keywords:
Cell deliveryElastin-like polypeptideInjectable hydrogelTemperature-responsive hydrogelThree-dimensional culture

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Polymer Chemistry

Background:

  • Injectable hydrogels are crucial for minimally invasive delivery of cells and bioactive compounds in tissue engineering.
  • Elastin-like polypeptides (ELPs) offer desirable properties like biocompatibility and biodegradability but face challenges in injectable hydrogel development due to hydrophobicity.
  • Coiled-coil unit-bound ELPs (CUBEs) were previously designed to overcome limitations of traditional ELPs for hydrogel formation.

Purpose of the Study:

  • To evaluate the injectability and cell delivery capabilities of a basic CUBE hydrogel system, O-CUBE (AVGVP)42-D88-CL.
  • To assess the in situ gelation behavior and cell viability within the O-CUBE hydrogel.
  • To demonstrate the potential of CUBE hydrogels for three-dimensional cell culture and delivery.

Main Methods:

  • Preparation of O-CUBE protein solution and mixing with human cervical cancer (HeLa) cells.
  • Injection of the cell-hydrogel mixture into pre-warmed culture medium (37 °C) to induce in situ gelation.
  • Assessment of gelation rate, cell morphology, cell-cell interactions, and cell proliferation using DNA assays.

Main Results:

  • O-CUBE hydrogel achieved approximately 90% gelation rate at 37 °C within pH 6-8.
  • Encapsulated HeLa cells formed spheroid structures, indicating facilitated cell-cell interactions in 3D.
  • HeLa cells encapsulated within the O-CUBE hydrogel demonstrated survival and proliferation.

Conclusions:

  • The O-CUBE hydrogel system exhibits excellent injectability and in situ gelation properties.
  • This CUBE-based hydrogel supports cell viability and proliferation, making it suitable for 3D cell culture.
  • The O-CUBE hydrogel system shows significant promise as a biomaterial for minimally invasive cell delivery in tissue engineering applications.