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Related Experiment Video

Updated: Sep 10, 2025

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Induction and characterization of oral submucous fibrosis model with different pathological stages in rats and mice.

Jianfei Tang1, Zekun Zhou1, Ge Wang1

  • 1Hunan Key Laboratory of Oral Health Research & Hunan 3D Printing Engineering Research Center of Oral Care & Hunan Clinical Research Center of Oral Major Diseases and Oral Health & Academician Workstation for Oral-maxilofacial and Regenerative Medicine & Xiangya Stomatological Hospital & Xiangya School of Stomatology, Central South University, Changsha, 410008, Hunan, China.

Scientific Reports
|August 24, 2025
PubMed
Summary

Researchers developed new animal models for oral submucous fibrosis (OSF) using bleomycin injections. These models mimic early and middle stages of OSF, aiding mechanistic studies and therapeutic development for this chronic disease.

Keywords:
Animal modelBleomycinHuman tissuesOral submucous fibrosis

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Area of Science:

  • Biomedical Research
  • Animal Modeling
  • Oral Pathology

Background:

  • Oral submucous fibrosis (OSF) is a chronic, progressive fibrotic disease.
  • Clinical stages of OSF are known, but lack of animal models impedes research.
  • Development of targeted interventions for OSF is hindered by limited mechanistic understanding.

Purpose of the Study:

  • To establish reliable rat and mouse models of OSF.
  • To simulate early and middle stages of fibrosis using varying bleomycin injection frequencies.
  • To provide tools for studying OSF mechanisms and developing stage-specific therapies.

Main Methods:

  • Induction of OSF in rats and mice via different bleomycin (BLM) injection frequencies (weekly vs. twice weekly).
  • Assessment of fibrosis characteristics: mucosal pallor, mouth opening limitation, collagen deposition, and inflammatory response.
  • Histological and molecular analyses to confirm stage-dependent changes and compare with human OSF tissues.

Main Results:

  • Both low-frequency and high-frequency BLM injections induced significant fibrosis and inflammation.
  • Histological and molecular analyses confirmed stage-dependent pathological changes.
  • High-frequency BLM injections resulted in more severe fibrosis and inflammation, mirroring human OSF stages.

Conclusions:

  • Stable and repeatable OSF models in rats and mice were successfully developed.
  • These models represent different pathological stages of OSF.
  • The models serve as valuable tools for OSF mechanistic studies and precise, stage-specific therapeutic development.