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Updated: Sep 10, 2025

Detection of Tissue-resident Bacteria in Bladder Biopsies by 16S rRNA Fluorescence In Situ Hybridization
Published on: October 18, 2019
Bifidobacterium longum can degrade the urinary N -glycoprotein Uromodulin
Elie Al Khoury1, Jean-Philippe Gourdine2,3
1Biochemistry & Molecular Biology, Lewis & Clark College, Portland, Oregon, United States of America.
Abstract:
Uromodulin (UMOD), the most abundant urinary glycoprotein, protects against urinary tract infections through glycan-mediated pathogen binding; nevertheless, its interactions with commensal bladder microbiota (urobiome) remain unexplored. Based on our previous bioinformatics analysis on the urobiome's capacity to digest host glycans with glycosyl hydrolase genes (GHs), we hypothesized that UMOD may serve as a nutrient source for selected bacteria. We cultured one of them, Bifidobacterium longum, in a minimal medium supplemented solely with purified UMOD. Our results indicate that UMOD is degraded by B. longum , supporting a new role for UMOD in the urobiome's metabolism.
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