Related Experiment Video
Updated: Sep 10, 2025
Software-Assisted Quantitative Measurement of Osteoarthritic Subchondral Bone Thickness
Published on: March 18, 2022
Interleukin-10 Mitigates Chondrocyte Ferroptosis Associated With Haemophilic Arthropathy via Modulation of the STAT3
Fudong Luo1,2, Yi Hu1, Yi Wang1
1Graduate School of Bengbu Medical University, Bengbu, Anhui, China.
Introduction:
Haemophilic arthropathy (HA) is characterized by recurrent intra-articular bleeding leading to cartilage degeneration. Ferroptosis plays a critical role in this process. While IL-10 is known to inhibit apoptosis, its effect on ferroptosis remains unexplored.
Aim:
To investigate IL-10's role in chondrocyte ferroptosis in homophilic arthropathy via STAT3 signalling.
Methods:
Cartilage samples from HA and OA patients were analyzed by TEM and Western blot for iron deposition and IL-10. ATDC5 chondrocytes were treated with FAC (iron overload), Erastin (ferroptosis inducer) and IL-10±STAT3 inhibitor (AG-490). Cell viability assays, Western blotting, reactive oxygen species detection and immunofluorescent staining were performed. Viability, ROS and pathway proteins were assessed.
Results:
HA chondrocytes exhibited elevated mitochondrial iron deposition and reduced IL-10 versus OA controls. IL-10 suppressed ferroptosis with efficacy comparable to Fer-1, mechanistically dependent on STAT3 activation.
Conclusion:
IL-10 suppresses ferroptosis in HA chondrocytes primarily via STAT3 signalling, providing theoretical support for its therapeutic potential for HA.
Related Concept Videos
01:20The JAK-STAT Signaling Pathway
01:24T Cell Types and Functions
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
01:01Regulation of Hematopoietic Stem Cells