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Updated: Sep 10, 2025
08:59
T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
Published on: January 12, 2021
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The canine T cell receptor repertoire
David Allaway1, Matt Harrison2, Claire Pink1
1Waltham, Petcare Science Institute, Leicestershire United Kingdom.
Immunohorizons
|August 25, 2025
Summary
Canine T cell receptor (TCR) repertoires are influenced by breed and individual genetics, showing stability over time. Age impacts TCR diversity, with puppies having more diverse repertoires than older dogs.
Area of Science:
- Immunology
- Genomics
- Veterinary Medicine
Background:
- TCR sequencing methods can be biased.
- Understanding canine TCR repertoires is crucial for immune monitoring.
Purpose of the Study:
- Develop and apply Tseek for unbiased TCR repertoire sequencing in dogs.
- Characterize TCR repertoire influences: individual, breed, and age.
- Evaluate TCR repertoires for immune status monitoring.
Main Methods:
- Utilized Tseek for minimal bias TCR sequencing.
- Analyzed RNA data from 32 dogs across 4 breeds and 50 Labrador Retrievers of varying ages.
- Identified V/J segments, annotated CDR3, and analyzed repertoire inferences.
Main Results:
- Canine TCR repertoires exhibit individual and breed-specific signatures.
- Repertoires remained stable for at least one year.
- Age-related effects observed: reduced diversity and increased high-frequency clones in older dogs; greater diversity in puppies.
Conclusions:
- Canine TCR repertoires contain stable, individual-specific information influenced by breed and age.
- Age-related decline in TCR diversity was noted.
- Further research is needed to understand the impact of these repertoire changes on canine immune function.
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