Phytochemical screening, antioxidant, and antimicrobial analysis of Portulaca oleracea seeds with in-silico molecular docking insights
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Khursheed Ahmad Sheikh1, Reyaz Hassan Mir2, Mohammad Ovais Dar3
1Pharmaceutical Chemistry Division, Department of Pharmaceutical Sciences, School of Applied Sciences and Technology, University of Kashmir, Hazratbal, Srinagar 190006 Kashmir, India; Pharmaceutical Chemistry, School of Pharmaceutical Education and Research (SPER), Jamia Hamdard, Hamdard Nagar, New Delhi, India.
Background:
Portulaca oleracea (PO), an annual succulent herb with global distribution, has been used medicinally since ancient times, earning the title "global panacea."
Aim:
This study aimed to perform phytochemical screening, antioxidant and antimicrobial analysis of PO seeds, including GCMS analysis of methanolic extract and molecular docking for antimicrobial mechanisms.
Materials And Methods:
PO seeds underwent phytochemical screening and methanolic extract analysis via DPPH, NO radical scavenging, and reducing power assays. Antimicrobial activity was tested against bacteria and fungi, with GCMS identifying compounds. Molecular docking was conducted via Autodock vina, against targets beta-tubulin (5FNV) and ABC transporter (6J9W).
Results:
Methanolic extract showed strong antioxidant activity (IC50: 125.2 µg/ml for DPPH, 402.89 µg/ml for NO) and concentration-dependent reducing power. It was highly effective against Saccharomyces cerevisiae and Hypochrea viridescens. GCMS identified colchicine, n-decanoic acid, and triepoxydecane, with colchicine showing high binding affinity to protein targets.
Conclusion:
The methanolic extract's potent antioxidant and antimicrobial effects, supported by colchicine's binding affinity, validate PO's medicinal potential, suggesting further therapeutic exploration.
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