To Spray or To Sublimate: Considerations for the Matrix Application of Three Common Positive Ion Mode Matrices
Ian Spears1, Gabriella Lagdameo1, Alyson Black2
1The Johns Hopkins University Applied Imaging Mass Spectrometry Core and Service Center; Division of Cancer Imaging Research; The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine; Baltimore, Maryland, 21205, United States.
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) matrix deposition methods impact crystal size and molecular detection. Sublimation minimizes crystal size but may reduce signal intensity for some lipids compared to spraying.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Mass Spectrometry
Background:
- Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) enables in situ molecular analysis.
- Effective matrix deposition is crucial for balancing analyte extraction and minimizing delocalization.
- Minimizing matrix crystal size is essential for high-resolution MALDI MSI.
Purpose of the Study:
- To compare pneumatic spraying and sublimation for matrix deposition in lipid imaging.
- To evaluate the effects of different matrices on crystal size, analyte delocalization, and signal intensity.
- To assess the impact of matrix deposition methods on lipid detection in biological tissues.
Main Methods:
- Comparison of a commercial automatic pneumatic sprayer and a sublimator for matrix deposition.
- Testing of three matrices: CHCA, DHAP, and DHB.
- Analysis of lipid imaging on mouse brain, heart, and kidney tissues.
Main Results:
- Matrix crystal size varied based on the matrix and deposition location (on or off tissue).
- Spraying led to increased delocalization for a subset of lipids.
- Sublimation resulted in decreased signal intensity for a subset of lipids.
Conclusions:
- Both spraying and sublimation have distinct effects on MALDI MSI lipid imaging.
- Matrix choice and deposition method influence crystal size, analyte delocalization, and signal detection.
- Optimizing matrix deposition is key for high-quality MALDI MSI lipid analysis.
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