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Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids
Published on: December 23, 2022
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The GNL3L-MDM2 Interaction Drives Esophageal Squamous Cell Carcinoma Progression
Aijie Yang1, Haiyun Song2, Yufeng Cheng3
1Department of Radiotherapy, Qilu Hospital of Shandong University (Qingdao), Qingdao, China.
Cancer Medicine
|August 26, 2025
Summary
Guanine nucleotide-binding protein-like 3-like (GNL3L) promotes esophageal squamous cell carcinoma (ESCC) progression by interacting with MDM2, affecting the p53-p21 pathway. GNL3L upregulation correlates with ESCC malignancy.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Esophageal squamous cell carcinoma (ESCC) is a significant global health concern.
- Understanding the molecular mechanisms driving ESCC progression is crucial for developing effective therapies.
Purpose of the Study:
- To investigate the role of GNL3L in ESCC progression.
- To elucidate the molecular mechanisms underlying GNL3L's influence on ESCC.
Main Methods:
- Immunohistochemistry to assess GNL3L expression in ESCC tissues.
- In vitro assays (EdU, CCK8, wound healing, Transwell, flow cytometry) to evaluate proliferation, migration, invasion, cell cycle, and apoptosis.
- qRT-PCR and western blot to analyze gene and protein expression (GNL3L, MDM2, p53, p21).
- In vivo tumor xenograft model in nude mice.
Main Results:
- GNL3L was significantly upregulated in ESCC tissues.
- GNL3L knockdown suppressed ESCC cell proliferation and migration, and induced apoptosis.
- GNL3L interacted with MDM2, modulating the MDM2-p53-p21 axis.
- In vivo studies showed that GNL3L knockdown significantly reduced tumor growth.
Conclusions:
- GNL3L is a key driver of ESCC progression and is associated with malignancy.
- The GNL3L-MDM2 interaction is critical for ESCC development.
- Targeting GNL3L may represent a potential therapeutic strategy for ESCC.
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