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Triplex DNA-Silver Nanoclusters: Tunable Fluorescence and Light-Enhanced Antibacterial Activity
Yuhan He1, Yuxuan Xue1, Xinyao Yi1
1Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, Hunan, P. R. China.
Researchers developed tunable fluorescent silver nanoclusters (AgNCs) using triple-stranded DNA (tsDNA). These tsDNA-AgNCs show enhanced antibacterial activity, with potential for biosensing and improved therapeutic treatments.
Area of Science:
- Nanotechnology
- Biochemistry
- Materials Science
Background:
- Silver nanoclusters (AgNCs) show promise for biosensing and antibacterial uses.
- Triple-stranded DNA (tsDNA) offers structural flexibility for novel material design.
Purpose of the Study:
- To construct tsDNA-AgNCs with tunable fluorescence and enhanced antibacterial properties.
- To investigate the mechanism of fluorescence enhancement and antibacterial activity.
Main Methods:
- Synthesizing AgNCs using cytosine-rich sequences on a tsDNA template.
- Tuning fluorescence emission by altering cytosine numbers in the DNA template.
- Evaluating antibacterial activity against Escherichia coli and Staphylococcus aureus.
- Assessing the effect of blue light irradiation on tsDNA-AgNCs' antibacterial capability.
Main Results:
- tsDNA-AgNCs exhibited significantly enhanced fluorescence compared to traditional AgNCs.
- Fluorescence color was tunable from red to green by modifying the DNA template.
- tsDNA-AgNCs demonstrated potent antibacterial activity against Gram-negative and Gram-positive bacteria.
- Blue light irradiation amplified antibacterial effects via increased reactive oxygen species (ROS) and silver ion (Ag+) release.
Conclusions:
- tsDNA-stabilized AgNCs represent a versatile platform for advanced biosensing applications.
- The developed tsDNA-AgNCs offer a promising strategy for enhanced antibacterial treatments.
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