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Related Experiment Video

Updated: Sep 10, 2025

Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
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Spatial isotope deep tracing deciphers inter-tissue metabolic crosstalk.

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This study introduces a new method to trace nutrient metabolism across organs, revealing complex metabolic crosstalk. The findings illuminate how tumors affect host metabolism and nutrient sharing between tissues.

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Area of Science:

  • Metabolomics
  • Systems Biology
  • Physiology

Background:

  • Organs collaborate to maintain metabolic homeostasis.
  • Spatial metabolomics can profile metabolic landscapes but not tissue crosstalk.
  • Isotope tracing is crucial for understanding metabolic pathways.

Purpose of the Study:

  • To develop a method for tracing nutrient metabolic fate in vivo.
  • To create a computational tool (MSITracer) for spatial metabolic analysis.
  • To investigate metabolic crosstalk and the impact of tumors on host metabolism.

Main Methods:

  • Utilized 13C-nutrients for comprehensive metabolic tracing.
  • Employed ambient mass spectrometry imaging-based isotope tracing.
  • Developed the MSITracer computational tool for spatial data analysis.

Main Results:

  • Characterized fatty acid crosstalk between liver and heart.
  • Mapped glutamine exchange across kidney, liver, and brain.
  • Disclosed tumor burden's influence on hexosamine biosynthesis pathway.
  • Identified lung-derived, glucose-fueled glutamine as a tumor glutamate source.

Conclusions:

  • The developed approach enables in situ metabolic activity characterization.
  • Facilitates interpretation of tissue metabolic communications in vivo.
  • Provides novel insights into organ-level metabolic interactions and disease states.