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Related Concept Videos

RNA Splicing01:32

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Alternative RNA Splicing02:18

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
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Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
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Chromatin Structure Regulates pre-mRNA Processing02:41

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
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Nonsense-mediated mRNA Decay02:27

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The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
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Related Experiment Video

Updated: Sep 10, 2025

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
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RNase III cleavage sites spread across splice junctions enforce sequential snoRNA processing.

Valérie Migeot1, Yves Mary1, Etienne Fafard-Couture2

  • 1URPHYM-GEMO, NARILIS, University of Namur, Namur, Belgium.

EMBO Reports
|August 26, 2025
PubMed
Summary
This summary is machine-generated.

Small nucleolar RNAs (snoRNAs) undergo a novel maturation process in fission yeast. An exon-exon junction hairpin recruits Pac1, an RNase III enzyme, to process downstream snoRNAs sequentially.

Keywords:
DroshaMaturationPac1RNAsesnoRNA

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Area of Science:

  • Molecular Biology
  • RNA Biology
  • Biochemistry

Background:

  • Small nucleolar RNAs (snoRNAs) are crucial non-coding RNAs in eukaryotes.
  • Precursor snoRNA transcript modifications (capping, polyadenylation) must be removed for function.
  • Maturation pathways for multi-snoRNA precursors are not fully understood.

Purpose of the Study:

  • Investigate a novel maturation mechanism for co-hosted snoRNA pairs.
  • Elucidate the role of exon-exon junction sequences in snoRNA processing.
  • Determine the evolutionary conservation of this maturation pathway.

Main Methods:

  • Studied snoRNA processing in Schizosaccharomyces pombe.
  • Analyzed precursor RNA structure and splicing events.
  • Identified protein factors involved in snoRNA maturation, including RNase III orthologs.

Main Results:

  • Discovered a new maturation mode for snoRNA pairs in adjacent intron/exon.
  • An exon-exon junction hairpin forms post-splicing, recruiting Pac1 (RNase III).
  • Pac1 cleaves the precursor, enabling downstream snoRNA maturation.

Conclusions:

  • Conditional RNase III cleavage at exon-exon junctions enforces sequential snoRNA processing.
  • This mechanism is conserved in animals and plants, suggesting evolutionary importance.
  • Reveals a novel layer of post-transcriptional gene regulation for snoRNAs.