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Related Experiment Video

Updated: Sep 10, 2025

Brain Pericyte Calcium and Hemodynamic Imaging in Transgenic Mice In Vivo

Brain Pericyte Calcium and Hemodynamic Imaging in Transgenic Mice In Vivo

Published on: November 20, 2021

Live Calcium Imaging on Mouse Primary Brain Endothelial Cells.

Dimitrios Spyropoulos¹, Jan Wenzel²

¹Institute of Experimental and Clinical Pharmacology and Toxicology, Center of Brain, Behavior and Metabolism (CBBM), University of Lübeck; DZHK (German Research Centre for Cardiovascular Research), Lübeck, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|August 26, 2025

Summary

This study presents a rapid method to measure calcium ion (Ca²⁺) signaling in brain endothelial cells. This technique aids in understanding cellular processes within an in vitro blood-brain barrier model.

Keywords:

Blood–brain barrier Chemical calcium indicators Fluo-4 Live calcium imaging Mouse brain endothelial cells

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Generation of an Immortalized Murine Brain Microvascular Endothelial Cell Line as an In Vitro Blood Brain Barrier Model

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Last Updated: Sep 10, 2025

Brain Pericyte Calcium and Hemodynamic Imaging in Transgenic Mice In Vivo

Brain Pericyte Calcium and Hemodynamic Imaging in Transgenic Mice In Vivo

Published on: November 20, 2021

Live Imaging of the Ependymal Cilia in the Lateral Ventricles of the Mouse Brain

Live Imaging of the Ependymal Cilia in the Lateral Ventricles of the Mouse Brain

Published on: June 1, 2015

Generation of an Immortalized Murine Brain Microvascular Endothelial Cell Line as an In Vitro Blood Brain Barrier Model

Generation of an Immortalized Murine Brain Microvascular Endothelial Cell Line as an In Vitro Blood Brain Barrier Model

Published on: August 29, 2012

Area of Science:

Cellular Biology
Neuroscience
Physiology

Background:

Calcium ions (Ca²⁺) act as vital intracellular second messengers in cellular signaling.
Intracellular calcium dynamics are critical for cardiovascular functions like vascular resistance and leukocyte transmigration.
Live calcium imaging offers high-resolution visualization of cellular calcium changes, essential for studying signaling pathways.

Purpose of the Study:

To develop a simple and fast method for investigating intracellular calcium changes.
To analyze calcium signaling in an in vitro blood-brain barrier model.
To utilize fluorescent dye Fluo-4 AM for calcium imaging in primary brain endothelial cells.

Main Methods:

Utilized an in vitro blood-brain barrier model.
Employed primary brain endothelial cells.
Applied the fluorescent dye Fluo-4 AM for calcium imaging.

Main Results:

Successfully established a method for investigating intracellular calcium changes.
Demonstrated the feasibility of using Fluo-4 AM in primary brain endothelial cells.
Provided a tool for studying calcium dynamics in a blood-brain barrier model.

Conclusions:

The described method is effective for studying intracellular calcium dynamics.
This technique facilitates research on cellular signaling in the blood-brain barrier.
The approach offers a valuable tool for neurovascular research.