Arbuscular mycorrhizal fungi colonization facilitates nitrogen uptake in cotton under nitrogen - reduction condition

Cotton is an economically important global crop, the yield and quality of which are strongly influenced by soil nitrogen. Low nitrogen use efficiency poses an important challenge to improve cotton yield and quality. The use of arbuscular mycorrhizal fungi (AMF) has been proposed as an effective solution to this challenge. Therefore, we conducted an indoor experiment using a compartmentalized culture system with cotton as the material and established three nitrogen treatments (1 g·kg^-1, 0.7 g·kg^-1, and 0 g·kg^-1) to investigate whether symbiosis between AMF and cotton roots could improve the nitrogen absorption capacity of cotton.

The results showed that under high-nitrogen, low-nitrogen, and nitrogen- free treatments, the contributions of AMF colonization to root NO₃⁻-N and NH₄⁺-N were 5.89%, 10.10%, 19.92% and 24.35%, 12.37%, 13.16% respectively. Furthermore, the symbiosis between AMF and roots promoted the absorption of soil NO₃⁻-N, NH₄⁺ -N, and dissolved organic nitrogen, and was beneficial for increasing the content of soil readily oxidizable carbon. Additionally, AMF colonization was significantly positively correlated with root tissue density, cotton biomass, and soil microbial activity, but significantly negatively correlated with soil total organic carbon.

Therefore, under nitrogen - reduction condition, roots will be more dependent on the contribution of mycelium to NO₃⁻-N, and AMF colonization was significantly positively correlated with root tissue density (P < 0.05), suggesting that mycelium may prolong its functional cycle by improving the root structure, thereby reducing the carbon and nitrogen consumption in host organ reconstruction. However, this mechanism needs to be further verified in combination with the direct measurement of root turnover rate.