Risk of cervical squamous cell carcinoma associated with a single nucleotide polymorphism in the RAD18 gene in the Chinese population and its significance as a predictive biomarker
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View abstract on PubMed
Summary
This summary is machine-generated.Genetic variations in the RAD18 gene, specifically rs250403 and rs615967, are linked to increased risk and poorer prognosis in cervical precancer (CIN III) and cervical squamous cell carcinoma (CSCC). These RAD18 polymorphisms may serve as predictive biomarkers for cervical cancer.
Area Of Science
- Genetics
- Oncology
- Molecular Biology
Background
- RAD18 is a key mismatch repair gene involved in post-replication repair, and its genetic variations are implicated in tumorigenesis.
- Cervical squamous cell carcinoma (CSCC) and its precursor, CIN III, represent significant health concerns globally.
Purpose Of The Study
- To investigate the association between RAD18 single nucleotide polymorphisms (SNPs) and the risk of CIN III and CSCC.
- To evaluate the correlation of RAD18 polymorphisms with clinicopathological features and prognosis in cervical cancer patients.
Main Methods
- Mismatch amplification PCR was used to analyze 6 RAD18 SNPs in 650 CIN III cases, 580 CSCC cases, and 1320 healthy controls.
- Haplotype analysis and correlation with clinical data, including prognostic indicators and RAD18 protein expression, were performed.
Main Results
- Specific genotypes (rs250403 GG, rs615967 GG) and alleles (G allele) of RAD18 were significantly associated with an increased risk of CIN III and CSCC.
- High-risk haplotypes (AG-GG, GG-AA, GG-AG, GG-GG) of RAD18 rs250403 and rs615967 were identified.
- RAD18 polymorphisms correlated with tumor differentiation, lymph node metastasis, vascular involvement, and decreased RAD18 protein expression in CSCC.
Conclusions
- Two RAD18 genetic polymorphisms (rs250403 and rs615967) are associated with both susceptibility and prognosis in CIN III and CSCC.
- Specific high-risk RAD18 haplotypes can potentially serve as genetic predictive biomarkers for cervical cancer.
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