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Updated: May 5, 2026
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Myelin Oligodendrocyte Glycoprotein MOG35-55 Induced Experimental Autoimmune Encephalomyelitis EAE in C57BL/6 Mice
Published on: April 15, 2014
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Elevated osteopontin reflects systemic inflammation in experimental autoimmune encephalomyelitis
Sungmoo Hong1, Kyungsook Jung2, Taeyoung Kang3
1College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju, Korea.
Anatomy & Cell Biology
|August 27, 2025
Summary
Osteopontin (OPN) levels increased in the blood and multiple organs of mice with experimental autoimmune encephalomyelitis (EAE). This suggests OPN may contribute to systemic autoimmune disorders.
Area of Science:
- Immunology
- Neuroscience
- Biochemistry
Background:
- Osteopontin (OPN) is a pro-inflammatory and immunomodulatory mediator.
- Its role in systemic disorders, particularly in autoimmune conditions like experimental autoimmune encephalomyelitis (EAE), requires further investigation.
Purpose of the Study:
- To examine the expression and localization of osteopontin (OPN) in various organs of mice with EAE.
- To determine the impact of EAE induction on OPN levels in blood and tissues.
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA) and western blot analysis were used to quantify OPN levels.
- Immunoreactivity was assessed in inflammatory and constitutive cell types across multiple organs.
Main Results:
- Serum OPN levels were significantly elevated in mice with EAE.
- OPN expression was upregulated in all examined tissues, including the liver, kidneys, intestines, and spinal cord.
- OPN immunoreactivity was observed in macrophages and enhanced in constitutive cell types.
Conclusions:
- Osteopontin is elevated systemically in EAE, indicating its involvement in the disease process.
- These findings suggest OPN's potential role as a key extracellular matrix component in systemic autoimmune disorders.
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