Related Experiment Video

Updated: Sep 10, 2025

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages
10:33

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

Published on: October 25, 2017

10.8K

Macrophage-derived reactive oxygen species promote Salmonella aggresome formation contributing to bacterial

Xiao Chen1, Kefan Fang1, Bo Li1

  • 1Biomedical Pioneering Innovation Center (BIOPIC), Peking-Tsinghua Center for Life Sciences, School of Life Sciences Peking University Beijing China.

Imeta
|August 27, 2025
PubMed

Insights

Macrophage reactive oxygen species (ROS) induce Salmonella aggresomes, leading to bacterial dormancy and antibiotic persistence. These dormant bacteria can revive when the environment improves, offering potential therapeutic targets.

Area of Science:

  • Microbiology
  • Immunology
  • Bacterial Pathogenesis

Background:

  • Phagocytosis by macrophages can induce a persistent, dormant state in Salmonella.
  • Reactive oxygen species (ROS) produced by macrophages play a role in this process.

Discussion:

  • Salmonella aggresomes, triggered by macrophage ROS, promote bacterial persistence.
  • Dormant Salmonella within aggresomes exhibit reduced ATP levels and metabolic activity.
  • Gene expression shifts, with upregulation of SPI-1 T3SS followed by SPI-2 T3SS, indicate adaptation to the host environment.

Key Insights:

  • Macrophage ROS are critical for Salmonella aggresome formation and subsequent dormancy.
  • Salmonella aggresomes facilitate escape from antibiotic treatment by entering a dormant state.
  • This dormancy is reversible, allowing bacteria to resuscitate when macrophage ROS production decreases.

Outlook:

  • Understanding Salmonella aggresome formation and dormancy could lead to novel strategies against antibiotic-resistant bacterial infections.
  • Targeting bacterial aggresomes may disrupt persistence mechanisms, enhancing antibiotic efficacy.

More Related Videos

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages11:10

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages

Published on: August 11, 2014

12.8K
High-throughput Screening of Chemical Compounds to Elucidate Their Effects on Bacterial Persistence07:25

High-throughput Screening of Chemical Compounds to Elucidate Their Effects on Bacterial Persistence

Published on: February 23, 2021

4.2K

Related Concept Videos

Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.5K
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
197
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
92
Stringent Response in E. coli01:23

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
52
Antibiotic Selection00:57

Antibiotic Selection

Overview
55.2K
Oxygen Requirements and Growth Patterns01:29

Oxygen Requirements and Growth Patterns

Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...
236