A computerized low-shear pendulum viscoelastometer, stress-relaxation, shear creep, and dynamic elastic moduli...

A computerized low-shear pendulum viscoelastometer that is well suited for measurements of the viscoelastic properties of soft biogels is described. The instrument has three modes of operation: (i) measurement of dynamic elastic moduli, (ii) stress relaxation, and (iii) shear creep-creep recovery. The main mechanical parts of the instrument include a specimen cuvette and a pendulum with a thin metal plate extending into the specimen cuvette. The cuvette and pendulum can undergo independent angular movements relative to a horizontal axis located about 12 cm above the specimen cuvette. The absolute angular position of the cuvette is under on-line computer control through a stepping motor driven micrometer. The angular position of the pendulum relative to the cuvette is measured using an inductive position detector. The necessary software for automatic operation, data acquisition and processing has been developed for all the three modes of operation. The instrument is well suited for measurements of dynamic elastic moduli in the range 0.02-150 dyn/cm2. Calibration data obtained using 100% ethanol are presented. The instrument suitability for measurements of stress relaxation and shear creep-creep recovery is illustrated by presenting the results of such measurements on a 5.5-mg/ml erythrocyte spectrin gel in isotonic solution.