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Updated: Sep 10, 2025
07:42
A Data-Driven Approach to Quantifying Immune States in Sepsis
Published on: February 7, 2025
313
The relationship between immune cell infiltration and necroptosis gene expression in sepsis: an analysis using
1Department of Pediatrics, Zhongnan Hospital of Wuhan University, Wuhan, China.
Frontiers in Cellular and Infection Microbiology
|August 27, 2025
Summary
This study reveals significant differences in necroptosis-related gene expression in sepsis, impacting immune cell activity and offering potential therapeutic targets for this life-threatening condition.
Area of Science:
- Molecular Biology
- Immunology
- Bioinformatics
Background:
- Sepsis is a life-threatening organ dysfunction driven by a dysregulated host response to infection.
- High mortality rates underscore the need for deeper understanding of sepsis mechanisms.
- This study investigates necroptosis-related gene expression and its impact on immune characteristics in sepsis.
Purpose of the Study:
- To identify differentially expressed necroptosis-related genes in sepsis.
- To analyze the functional enrichment of these genes in biological pathways.
- To explore the relationship between necroptosis-related genes and immune cell infiltration in sepsis.
Main Methods:
- Utilized gene expression profiles and single-cell RNA sequencing data from the GEO database.
- Applied limma for differential gene expression analysis and clusterProfiler for GO/KEGG enrichment.
- Employed GSVA, GSEA, ssGSEA, Seurat, and SingleR for pathway and immune cell analysis.
Main Results:
- Identified 849 necroptosis-related differentially expressed genes (DEGs), with 843 upregulated in sepsis.
- LASSO regression pinpointed 22 key DEGs, including CTSS, MAPK8, and MPRIP.
- Found significant enrichment in apoptotic and NF-κB signaling pathways, altered Wnt signaling, and distinct immune cell infiltration patterns, particularly in B and T cells.
Conclusions:
- Integrated bioinformatics analysis reveals the role of necroptosis-related genes in sepsis pathogenesis.
- Findings highlight the influence of these genes on immune responses.
- This research may inform future therapeutic strategies targeting necroptosis pathways in sepsis.
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