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Updated: Sep 10, 2025

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
Published on: April 7, 2014
Phase-contrast imaging of a dense atomic cloud
M Frometa1,2, P G Santos Dias3, P H Nantes Magnani3
1Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos SP 13566-970, Brazil.
None:
We demonstrate that phase-contrast imaging (PCI) can reliably reconstruct the in situ density profile even for highly spatially and optically dense samples. In our experiment, we achieve high spatial densities of up to 7.9 × 1013 atoms/cm3 and optical depths up to 64 in a dense cold atomic cloud of 88Sr atoms. The use of a spatial light modulator instead of a fixed phase plate in the PCI setup provides enhanced flexibility and control of imaging parameters, making this imaging technique robust against imaging artifacts and adaptable to varying experimental conditions. Moreover, we quantify the conditions under which the standard single-atom polarizability model remains accurate for PCI in a density regime where collective effects should modify the atomic response of the system. We experimentally validate our statements by showing excellent agreement with time-of-flight measurements even at the highest densities. Our results establish PCI as a reliable and versatile method for characterizing spatially dense atomic clouds.
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