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Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse

Published on: March 8, 2013

Surface-Engineered HA-PEG-ICG/PLGA Nanoprobes with Vessels Targeting for Lymphatic System Visualization.

Hao-Han Chiang¹, Yu-Teng Chang^2,3, Wei-Ren Huang¹

¹Department of Materials Science and Engineering, Feng Chia University, Taichung 40724, Taiwan.

ACS Applied Bio Materials

|August 27, 2025

Summary

We developed targeted nanoparticles for enhanced lymphatic imaging. These nanoparticles show promise for early lymphedema diagnosis and real-time lymphatic mapping.

Keywords:

ICG/PLGA nanocarrier hyaluronic acid indocyanine green lymphatic imaging lymphedema diagnostics targeted fluorescence probe

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Area of Science:

Biomedical Engineering
Nanotechnology
Medical Imaging

Background:

Targeted imaging of the lymphatic system is crucial for diagnosing and managing lymphatic disorders like lymphedema.
Current imaging methods may lack specificity and efficiency for detailed lymphatic visualization.

Purpose of the Study:

To develop a novel lymphatic-targeted fluorescent nanoprobe for improved lymphatic imaging.
To evaluate the safety, targeting efficiency, and imaging capabilities of the developed nanoprobe.

Main Methods:

Synthesis of hyaluronic acid-polyethylene glycol (HA-PEG) surface-modified poly(lactic-co-glycolic acid) (PLGA) nanoparticles encapsulating indocyanine green (ICG) (HA-PEG-ICG/PLGA NPs) via microemulsion.
Characterization using UV-vis spectroscopy, fluorescence analysis, FTIR, and ζ-potential measurements.
In vitro biocompatibility assays with human keratinocytes and mouse lymphatic endothelial cells, and in vivo imaging studies in mice.

Main Results:

Physicochemical characterization confirmed nanoparticle stability and successful functionalization.
In vitro studies demonstrated excellent biocompatibility and enhanced cellular uptake in lymphatic endothelial cells via HA-mediated receptor binding (LYVE-1).
In vivo imaging revealed prolonged retention and selective fluorescence accumulation in lymphatic vessels, outperforming free ICG and non-targeted nanoparticles.

Conclusions:

HA-PEG-ICG/PLGA NPs represent a safe and effective nanoplatform for real-time lymphatic imaging.
The targeted system shows significant potential for early lymphedema diagnosis and intraoperative lymphatic mapping.
This approach could be integrated into future theranostic strategies for lymphatic diseases.