Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Type IV Collagen of Basal Lamina

Type IV Collagen of Basal Lamina

Type IV collagen is a 400 nm long, network-forming collagen that acts as a barrier between the epithelial and endothelial cells. Type IV collagen forms the backbone of the basement membrane by scaffolding with laminin, entactin, proteoglycans, and fibronectin. Apart from rendering structural support to the basement membrane, it also helps entail signaling potentials necessary for both pathological and physiological functions.
A type IV collagen molecule has six alpha chains which can exist in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Critical-Size Muscle Defect Regeneration Using an Injectable Cell-Laden Nanofibrous Matrix: An Ex Vivo Mouse Hindlimb Organ Culture Study.

International journal of molecular sciences·2025

Same author

Protocol for quantifying the horizontal and vertical distribution of junctional proteins in fixed epithelial cells.

STAR protocols·2025

Same author

Quantification of horizontal and vertical distribution of junctional proteins in fixed epithelial cells.

bioRxiv : the preprint server for biology·2025

Same author

Synergetic role of TRPV4 inhibitor and mechanical loading on reducing inflammation.

Frontiers in immunology·2025

Same author

Tideglusib enhances ALP activity and upregulates RANKL expression in Osteoblast-macrophage Co-cultures within a 3D collagen scaffold.

Journal of dentistry·2024

Same author

The Scribble-SGEF-Dlg1 complex regulates E-cadherin and ZO-1 stability, turnover and transcription in epithelial cells.

Journal of cell science·2024

Search research articles

Related Experiment Video

Updated: Jun 7, 2026

Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules

Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules

Published on: August 19, 2015

Immunomodulation Through Fibroblast-Derived Extracellular Vesicles (EVs) Within 3D Polycaprolactone-Collagen Matrix.

Afsara Tasnim¹, Diego Jacho¹, Agustin Rabino²

¹Department of Bioengineering, University of Toledo, Toledo, OH 43606, USA.

Biomimetics (Basel, Switzerland)

|August 27, 2025

Summary

Human fibroblast extracellular vesicles (EVs) in injectable scaffolds reprogram macrophages to reduce inflammation and maintain tissue engineering scaffold integrity. This cell-free approach enhances regenerative medicine potential.

Keywords:

collagen extracellular vesicles fibroblasts immunomodulation inflammation macrophages polycaprolactone tissue regeneration

More Related Videos

Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms

Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms

Published on: April 11, 2017

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay

Published on: December 26, 2019

Related Experiment Videos

Last Updated: Jun 7, 2026

Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules

Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules

Published on: August 19, 2015

Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms

Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms

Published on: April 11, 2017

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay

Published on: December 26, 2019

Area of Science:

Biomaterials Science
Regenerative Medicine
Immunomodulation

Background:

Extracellular vesicles (EVs) are acellular tools for immune modulation in tissue engineering.
Human fibroblast-derived EVs show potential for therapeutic applications.
Scaffold integrity and immune response are critical for regenerative therapies.

Purpose of the Study:

To investigate fibroblast-derived EVs within a 3D injectable scaffold (PNCOL) for modulating macrophage behavior.
To assess the impact of EVs on scaffold integrity under inflammatory conditions.
To evaluate the potential of this cell-free approach for regenerative medicine.

Main Methods:

Isolation and characterization of human fibroblast-derived EVs.
Preparation of macrophage-laden PNCOL scaffolds with different conditions (MP, F-MP, EV-MP).
Assessment of scaffold structure (SEM, Masson's trichrome) and immunomodulatory effects (metabolic assays, gene expression, IHC for M1/M2 markers).

Main Results:

Fibroblast-derived EVs preserved scaffold structure and enhanced macrophage metabolic activity.
EV-encapsulated scaffolds showed upregulation of anti-inflammatory markers (TGF-β, CD163, CCL18) and CD206.
A phenotypic shift towards M2-like macrophages was observed in EV-encapsulated scaffolds.

Conclusions:

Fibroblast-derived EVs in injectable PCL-collagen scaffolds offer a cell-free method to modulate inflammation.
This strategy preserves scaffold structure and supports regenerative healing.
The approach shows promise for immuno-instructive platforms in regenerative medicine.