Exploring the Rheological Properties of 3D Bioprinted Alginate-Based Hydrogels for Tissue Engineering

The development of alginate/polyacrylamide hydrogels for various biomedical applications has attracted significant interest, particularly due to their potential use in wound healing and tissue engineering. This study explores the fabrication of these hydrogels via 3D bioprinting with ultraviolet light curing, focusing on how the alginate concentration and curing speed impact their mechanical properties. Rheological testing was employed to examine the viscoelastic behavior of alginate/polyacrylamide hydrogels manufactured using a 3D bioprinting technique. The relaxation behavior and dynamic response of these hydrogels were analyzed under torsional stress, with relaxation curves fitted using a two-term Prony series. Fourier Transform Infrared (FTIR) spectroscopy was also employed to assess biocompatibility and the conversion of acrylamide. This study successfully demonstrated the printability of alginate/polyacrylamide hydrogels with varying alginate contents. The rheological results indicated that 3D bioprinted hydrogels exhibited significantly high stiffness, viscoelasticity, and long relaxation times. The curing speed had a minimal impact on these properties. Additionally, the FTIR analysis confirmed the complete conversion of polyacrylamide, ensuring no harmful effects in biological applications. The study concludes that 3D bioprinting significantly enhances the mechanical properties of alginate/polyacrylamide hydrogels, with the alginate concentration playing a key role in the shear modulus. These hydrogels show promising potential for biocompatible applications such as wound healing dressings.