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Related Concept Videos

Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

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Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
314

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Related Experiment Video

Updated: Sep 10, 2025

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
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Microfluidic Platforms for Ex Vivo and In Vivo Gene Therapy.

Sungjun Kwak1, Hyojeong Lee1, Dongjun Yu1

  • 1Department of Biological Sciences and Bioengineering, Inha University, Incheon 22212, Republic of Korea.

Biosensors
|August 27, 2025
PubMed
Summary

Microfluidic platforms offer advanced solutions for nucleic acid therapeutics (NATs) delivery. These integrated systems unify cellular manipulation and tissue targeting for scalable, programmable, and clinically translatable gene therapy.

Keywords:
gene therapymicrofluidicsnucleic acid-based therapeutics

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Area of Science:

  • Biotechnology
  • Biomedical Engineering
  • Molecular Therapy

Background:

  • Nucleic acid therapeutics (NATs) show clinical promise but face delivery challenges.
  • Efficient and scalable delivery is crucial for ex vivo and in vivo gene therapy.
  • Microfluidics offers precise control for intracellular delivery and carrier fabrication.

Purpose of the Study:

  • To review microfluidic strategies for cellular gene delivery.
  • To discuss carrier synthesis for systemic administration.
  • To highlight integrated microfluidic systems unifying ex vivo and in vivo delivery.

Main Methods:

  • Mechanoporation, electroporation, and sonoporation for cellular delivery.
  • Synthesis of lipid nanoparticles, polymeric particles, and extracellular vesicles.
  • Development of integrated microfluidic systems for combined delivery and engineering.

Main Results:

  • Microfluidics enables precise intracellular delivery and consistent carrier fabrication.
  • Integrated systems streamline workflows from cell manipulation to tissue targeting.
  • Examples include delivering genetic material to EV-secreting cells and hydrogel encapsulation.

Conclusions:

  • Integrated microfluidic platforms are fundamental for next-generation NATs.
  • These platforms facilitate scalable, programmable, and clinically translatable gene therapy.
  • The convergence of gene delivery and carrier engineering is key to advancing NATs.