An Amperometric Enzyme-Nanozyme Biosensor for Glucose Detection
Asta Kausaite-Minkstimiene1, Aiste Krikstaponyte1, Nataliya Stasyuk2
1NanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko st. 24, LT-03225 Vilnius, Lithuania.
Biosensors
|August 27, 2025
Summary
A new amperometric glucose biosensor using platinum cobalt nanoparticles and glucose oxidase enzyme offers sensitive and selective detection of blood glucose. This development shows promise for accurate glucose monitoring in diabetic patients.
Area of Science:
- Electrochemistry
- Nanomaterials Science
- Biomedical Engineering
Background:
- Amperometric biosensors are crucial for medical diagnostics, particularly for blood glucose monitoring in diabetes.
- Existing biosensors face challenges in sensitivity, stability, and interference.
Purpose of the Study:
- To develop a highly sensitive and stable amperometric glucose biosensor.
- To utilize bimetallic platinum cobalt nanoparticles for enhanced electrocatalytic activity.
Main Methods:
- Immobilized glucose oxidase (GOx) on a graphite rod electrode modified with platinum cobalt (PtCo) nanoparticles.
- Electrocatalysis of H2O2 reduction by PtCo nanoparticles.
- Amperometric detection of glucose concentration.
Main Results:
- The biosensor showed a linear response to glucose from 0.04-2.18 mM.
- Achieved high sensitivity (19.38 μA mM⁻¹ cm⁻²), low detection limit (0.021 mM), and quantification limit (0.064 mM).
- Demonstrated excellent repeatability, stability, selectivity, and accuracy in blood serum samples.
Conclusions:
- The developed PtCo nanoparticle-based amperometric glucose biosensor is highly sensitive, selective, and stable.
- It shows significant potential for accurate glucose determination in real-world clinical applications.
- This biosensor offers a promising advancement in diabetic patient monitoring.
