Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Liver Regeneration01:24

Liver Regeneration

3.4K
The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
3.4K
Introduction to Fibroblasts01:09

Introduction to Fibroblasts

3.2K
Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Improving Generalizability in Whole-Cell Antibiotic Discovery Through Active Learning.

bioRxiv : the preprint server for biology·2026
Same author

The emergence of novel versus known three-dimensional structures from random sequences.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

AEGIS reveals epitope- and clone-resolved convergence of CNS B and T cell autoreactivity in ROHHAD.

bioRxiv : the preprint server for biology·2026
Same author

HuR Regulates GATA3-Driven Type 2 Inflammation in CD4<sup>+</sup> T cells and ILC2 in Airway Inflammation.

bioRxiv : the preprint server for biology·2026
Same author

NCOR2 represses MHC class I molecule expression to drive metastatic progression of breast cancer.

Nature communications·2026
Same author

Design of an ultrabright biosensor for dynamic imaging of kinase activity in cells.

Proceedings of the National Academy of Sciences of the United States of America·2026

Related Experiment Video

Updated: Sep 10, 2025

3D Imaging of the Liver Extracellular Matrix in a Mouse Model of Non-Alcoholic Steatohepatitis
06:46

3D Imaging of the Liver Extracellular Matrix in a Mouse Model of Non-Alcoholic Steatohepatitis

Published on: February 25, 2022

2.0K

Resolving fibrosis by stimulating HSC-dependent extracellular matrix degradation.

Sachin Sharma1, Vijaya Prathigudupu1, Carson Cable1

  • 1Division of Gastroenterology and Hepatology, Department of Medicine, University of California, San Francisco, CA 94143, USA.

Science Translational Medicine
|August 27, 2025
PubMed
Summary

Inhibiting acid ceramidase (aCDase) boosts ceramide, promoting extracellular matrix (ECM) degradation and resolving liver fibrosis. This uncovers a new therapeutic strategy targeting ECM breakdown for fibrosis treatment.

More Related Videos

Seven Steps to Stellate Cells
06:40

Seven Steps to Stellate Cells

Published on: May 10, 2011

34.0K
Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis
08:56

Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis

Published on: February 10, 2015

52.8K

Related Experiment Videos

Last Updated: Sep 10, 2025

3D Imaging of the Liver Extracellular Matrix in a Mouse Model of Non-Alcoholic Steatohepatitis
06:46

3D Imaging of the Liver Extracellular Matrix in a Mouse Model of Non-Alcoholic Steatohepatitis

Published on: February 25, 2022

2.0K
Seven Steps to Stellate Cells
06:40

Seven Steps to Stellate Cells

Published on: May 10, 2011

34.0K
Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis
08:56

Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis

Published on: February 10, 2015

52.8K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Hepatology

Background:

  • Tissue fibrosis results from extracellular matrix (ECM) imbalance.
  • Current fibrosis treatments focus on reducing ECM production, not degradation.
  • Hepatic stellate cells (HSCs) play a key role in ECM regulation.

Purpose of the Study:

  • To investigate the role of acid ceramidase (aCDase) and ceramide in ECM degradation.
  • To identify signaling pathways involved in ECM breakdown in HSCs.
  • To explore aCDase as a therapeutic target for liver fibrosis.

Main Methods:

  • Investigated signaling pathways in primary human HSCs.
  • Utilized genetic reduction and pharmacological inhibition of aCDase.
  • Employed carbon tetrachloride (CCl4) and FPC mouse models of liver fibrosis.
  • Analyzed ceramide signaling in patients with metabolic dysfunction-associated steatotic liver disease.

Main Results:

  • Inhibition of aCDase increased ceramide levels in HSCs.
  • PKCα activation and ERK1/2 signaling induced MMP-1, promoting ECM degradation.
  • Reduced aCDase activity led to collagen degradation and fibrosis resolution in mouse models.
  • Ceramide signaling correlated with ECM remodeling in human liver disease patients.

Conclusions:

  • Ceramide signaling is a key regulator of ECM degradation.
  • aCDase inhibition promotes ECM breakdown and fibrosis resolution.
  • aCDase is a potential therapeutic target for reversing liver fibrosis.