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Retinal proteomic analysis reveals ON/OFF visual stimulation-specific changes in a Guinea pig myopia model
Qin Dai1, Qiqi Xie2, Frank Schaeffel3
1State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital, Wenzhou Medical University, Wenzhou, 325027, China; National Clinical Research Center for Ocular Diseases, Eye Hospital, Wenzhou Medical University, Wenzhou, 325027, China.
Experimental Eye Research
|August 27, 2025
Summary
Artificial dynamic ON/OFF light stimulation effectively suppressed myopia development in guinea pigs. This visual stimulation also altered retinal proteomic profiles, suggesting new therapeutic targets for myopia control.
Area of Science:
- Ophthalmology
- Proteomics
- Animal Models
Background:
- Myopia is a growing global health concern.
- Current myopia control strategies have limitations.
- Understanding the molecular mechanisms of myopia is crucial for developing new treatments.
Purpose of the Study:
- To investigate the impact of artificial dynamic ON/OFF light stimulation on myopia development in guinea pigs.
- To analyze associated retinal proteomic changes and elucidate underlying mechanisms.
- To identify potential therapeutic targets for myopia intervention.
Main Methods:
- Myopia was induced using -4 diopter lenses in guinea pigs.
- Animals were exposed to balanced ON/OFF (natural control, NC), ON, or OFF stimulation for 7 days.
- Axial length, refraction, choroidal thickness, and retinal proteome (LC-MS/MS) were analyzed.
Main Results:
- ON and OFF stimulation reduced myopia compared to NC.
- NC myopic eyes showed thinner choroids; ON/OFF groups had no difference.
- 646 differentially expressed proteins (DEPs) were identified, affecting glycometabolism and RNA function.
- The Rap1 signaling pathway was downregulated in ON/NC groups.
- Common DEPs implicated in myopia are linked to oxidative stress and inflammation.
Conclusions:
- Dynamic ON/OFF light stimulation suppresses myopia progression in guinea pigs.
- Specific retinal proteomic changes, including glycometabolism and RNA function alterations, are associated with this effect.
- The Rap1 signaling pathway and proteins involved in oxidative stress/inflammation are potential targets for myopia intervention.
