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Summary

We developed a non-invasive optical method using Fluorescence Lifetime Imaging Microscopy (FLIM) to monitor paper oxidation. This technique accurately detects paper degradation, crucial for preserving cultural heritage materials.

Keywords:
FLIMLabel-free fluorescence analysisNon-invasive analysisPaper ageing

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Heritage Science

Background:

  • Paper ageing is a complex, irreversible process affecting diverse materials.
  • Current analytical techniques for paper deterioration have limitations in sensitivity, resolution, and applicability to historical artifacts.
  • Non-invasive methods are crucial for analyzing sensitive materials like cultural heritage objects.

Purpose of the Study:

  • To present a novel, fully optical, non-invasive method for monitoring paper oxidation.
  • To demonstrate the utility of Fluorescence Lifetime Imaging Microscopy (FLIM) for assessing paper degradation.
  • To establish a new diagnostic approach for sensitive materials.

Main Methods:

  • Utilized label-free Fluorescence Lifetime Imaging Microscopy (FLIM).
  • Measured intrinsic fluorescence lifetime changes in paper samples.
  • Correlated FLIM data with Fourier-Transform Infrared Spectroscopy (FTIR) indicators of ageing.
  • Validated the method on modern and historical paper samples under controlled oxidation.

Main Results:

  • Intrinsic fluorescence lifetime of paper demonstrably changes with oxidation.
  • Observed strong correlations between fluorescence lifetime shifts and FTIR indicators of paper ageing.
  • Successfully validated the non-invasive FLIM approach on diverse paper samples.

Conclusions:

  • The presented FLIM method offers a sensitive, non-invasive means to monitor paper oxidation.
  • This technique provides a valuable tool for assessing the degradation of paper-based materials, especially in heritage science.
  • Represents a significant advancement toward new diagnostic methodologies for material preservation.