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Optimization and Characterization of Bioactive Metabolites from Cave-Derived Rhodococcus jialingiae C1
Muhammad Rafiq1,2, Umaira Bugti2, Muhammad Hayat3
1Guizhou Key Laboratory of Microbiome and Infectious Disease Prevention and Control, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education of Guizhou, School of basic Medical Sciences, Joint Laboratory of Shanghai Dongli One Health Research Institute Co., Ltd., Guizhou Medical University One Health Research Institute, Gui'an 561113, China.
Biomolecules
|August 28, 2025
Summary
Extremophilic bacteria from Pakistan
Area of Science:
- Microbiology
- Biotechnology
- Natural Products Chemistry
Background:
- Extremophilic microorganisms are a promising source of novel bioactive compounds.
- Antibiotic resistance necessitates the discovery of new antimicrobial agents.
- Cave environments harbor unique microbial communities with potential therapeutic applications.
Purpose of the Study:
- To isolate and characterize extremophilic bacteria from a cave environment.
- To evaluate the antibacterial and antioxidant potential of isolated bacteria.
- To identify and characterize novel bioactive metabolites produced by promising isolates.
Main Methods:
- Bacterial isolation from Chamalang cave samples.
- Antibacterial and antioxidant activity assays (DPPH radical scavenging).
- Optimization of culture conditions (media, pH, temperature, nutrient sources).
- Metabolite profiling using High-Performance Liquid Chromatography (HPLC) and Quadrupole Time-of-Flight Mass Spectrometry (QTOF-MS).
Main Results:
- Isolation of Rhodococcus jialingiae (isolate C1) with significant activity against multidrug-resistant pathogens (MDR).
- Demonstrated potent antioxidant activity (71% DPPH radical scavenging).
- Identified novel metabolites including phenolic compounds, Streptolactam C, Puromycin, and a putative aromatic polyketide.
- Optimized conditions significantly enhanced biomass and metabolite yields.
Conclusions:
- Rhodococcus jialingiae isolate C1 is a valuable source of novel antimicrobials and antioxidants.
- Environmental adaptation of extremophiles correlates with unique metabolic potential.
- This discovery supports natural product discovery pipelines for combating antibiotic resistance.
