A Scoping Review on Salivary Oxytocin and Vasopressin Measurement in the Dog

Oxytocin (OT) and vasopressin (AVP) are attracting interest as potential measures for dog welfare and human-dog interactions. The potential for detection and measurement of these two neuropeptides in canine saliva provides a viable alternative to more invasive collection methods. This scoping review aims to analyse the scientific literature measuring salivary OT and AVP in dogs, the methods employed, and the areas of research. A comprehensive search was conducted on the Web of Science, Scopus, and ProQuest platforms, yielding a selection of peer-reviewed, primary research studies that specifically measured dog salivary OT and/or AVP. The final selection comprised 16 articles from 2017 to 2024, offering insights into the methods employed for the collection, processing, and analysis of dog saliva samples. The findings highlighted significant variability in OT and AVP salivary concentrations, which also depended on the methods employed. The research areas of the included articles were primarily linked to the social and maternal behaviour spheres. This study draws from extant research to present an overview of the challenges associated with measuring salivary OT and AVP in dogs. It provides a comprehensive picture of this multifaceted issue, which is crucial for accurately interpreting these peptides as indicators of dog welfare.