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Camellia japonica Flower Extract and the Active Constituent Hyperoside Repair DNA Damage Through FUNDC1-Mediated

Hongqi Gao1,2, Jiahui Shi1, Guangtao Li2

  • 1School of Pharmacy, Guangdong Engineering Technology Research Centre of Molecular Probe and Biomedicine Imaging, Guangdong Pharmaceutical University, Guangzhou 510006, China.

Antioxidants (Basel, Switzerland)
|August 28, 2025
PubMed

Insights

Camellia japonica flower extract and hyperoside combat skin aging by enhancing mitochondrial autophagy. This process improves cellular energy and skin barrier function, offering a novel anti-aging strategy.

Area of Science:

  • Dermatology
  • Cell Biology
  • Biochemistry

Background:

  • Skin aging is linked to mitochondrial dysfunction and cell cycle issues.
  • Targeting mitochondrial quality control is a key area for anti-aging research.

Purpose of the Study:

  • To investigate the anti-aging effects of Camellia japonica flower (CJF) extract and hyperoside.
  • To elucidate the mechanism of CJF extract and hyperoside in a doxorubicin-induced senescence model in human skin fibroblasts (HSFs).

Main Methods:

  • Doxorubicin (DOX)-induced endogenous senescence model in HSFs.
  • LC-MS proteomics analysis.
  • Assessment of mitochondrial membrane potential, reactive oxygen species (ROS) levels, cell cycle progression, and senescence-associated β-galactosidase (SA-β-gal) activity.

Main Results:

  • CJF extract and hyperoside activated the FUNDC1-mediated mitochondrial autophagy pathway.
  • They improved mitochondrial function, reduced ROS accumulation, and alleviated cell cycle arrest and SA-β-gal expression.
  • Enhanced mitochondrial autophagy restored energy metabolism, promoted collagen and elastin synthesis, and repaired skin barrier function (Claudin 1).

Conclusions:

  • CJF extract and hyperoside delay skin aging by regulating the FUNDC1-dependent mitochondrial autophagy pathway.
  • This study provides a theoretical basis for developing new anti-aging agents targeting mitochondrial quality control.
Keywords:
Camellia japonica flower extractDNA damageFUNDC1-mediated mitophagy pathwayhyperosideskin anti-aging

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