Jove
Visualize
Contact Us

Related Concept Videos

Antibiotic Selection00:57

Antibiotic Selection

55.2K
Overview
55.2K
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

197
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
197
Genomic DNA in Prokaryotes00:46

Genomic DNA in Prokaryotes

44.5K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
44.5K
Global Regulatory Systems01:28

Global Regulatory Systems

71
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
71

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biphasic Metabolic Control of Shikimic Acid Production in <i>E. coli</i> via an Antisense RNA.

ACS omega·2026
Same author

Early Warning Signals in Ecological Time-Series.

Entropy (Basel, Switzerland)·2026
Same author

Identification of Opportunistic Pathogens on the Skin of Salamanders for Use as Molecular Targets of a De Novo Design of Multitarget Anti-<i>Bd</i> Proteins.

International journal of microbiology·2026
Same author

Probiotic and Postbiotic Interactions of <i>Lactobacillus</i> Strains with <i>Candida albicans</i>: Antifungal Effects Through Microbial Competition.

Antibiotics (Basel, Switzerland)·2026
Same author

Stress responses and dynamic equilibrium: Key determinants of aging in the <i>C. elegans clk-1</i> mutant.

bioRxiv : the preprint server for biology·2025
Same author

Numerical Modeling of Gentamicin Transport in Agricultural Soils: Implications for Environmental Pollution.

Antibiotics (Basel, Switzerland)·2025
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Sep 10, 2025

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
09:00

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Published on: May 2, 2018

11.9K

Deciphering Common Genetic Pathways to Antibiotic Resistance in Escherichia coli Using a MEGA-Plate Evolution System.

Nami Morales-Durán1,2, Angel León-Buitimea1,2, Roberto Álvarez Martínez3

  • 1Facultad de Ciencias Químicas, Universidad Autónoma de Nuevo León (UANL), San Nicolás de los Garza 66455, Mexico.

Antibiotics (Basel, Switzerland)
|August 28, 2025
PubMed
Summary

Antimicrobial resistance (AMR) in Escherichia coli shows varied evolutionary paths under different antibiotics. Targeting shared resistance genes may offer new therapeutic strategies.

Keywords:
Escherichia coli evolutionMEGA-plate systemOne Health approachantibiotic resistance genes (ARGs)antimicrobial resistance (AMR)

More Related Videos

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach
12:32

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Published on: December 14, 2019

14.1K
Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

46.6K

Related Experiment Videos

Last Updated: Sep 10, 2025

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
09:00

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance

Published on: May 2, 2018

11.9K
Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach
12:32

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Published on: December 14, 2019

14.1K
Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

46.6K

Area of Science:

  • Microbiology
  • Evolutionary Biology
  • Genomics

Background:

  • Antimicrobial resistance (AMR) is a critical global health challenge.
  • Understanding bacterial adaptation mechanisms is crucial for combating AMR.

Purpose of the Study:

  • Investigate genotypic and phenotypic evolution of *Escherichia coli* under meropenem and gentamicin selection.
  • Compare these evolutionary trajectories with florfenicol-resistant strains.
  • Utilize a modified Microbial Evolution and Growth Arena (MEGA-plate) system for real-time adaptation tracking.

Main Methods:

  • Employing a 40 × 50 cm MEGA-plate system for controlled *E. coli* evolution experiments.
  • Incubating cultures at 37 °C for 9-13 days to observe adaptation across antibiotic gradients.
  • Conducting comprehensive genomic analysis to identify resistance loci and regulatory networks.

Main Results:

  • Meropenem induced significant genetic heterogeneity and morphological changes in *E. coli*, linked to SOS response.
  • Gentamicin resulted in uniform resistance profiles with minimal morphological alterations.
  • A core resistome of 22 chromosomal loci was identified across all antibiotic exposures, with *baeR*, *gadX*, and *marA* playing key regulatory roles.

Conclusions:

  • *E. coli* exhibits diverse adaptive strategies, including metabolic flexibility and regulatory network activation, to overcome antibiotic pressure.
  • The MEGA-plate system is effective for studying AMR dynamics in a controlled environment.
  • Targeting shared regulatory pathways could be a promising strategy to combat AMR and preserve antibiotic efficacy.