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Related Concept Videos

Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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Deep Learning-Based Imaging Analysis Reveals Radiation-Induced Bystander Effects on Cancer Cell Migration and the

Ryosuke Seino1, Hisanori Fukunaga1

  • 1Department of Biomedical Science and Engineering, Faculty of Health Sciences, Hokkaido University, N12 W5 Kita-ku, Sapporo 060-0812, Japan.

International Journal of Molecular Sciences
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Chemoradiotherapy can unexpectedly increase cancer cell migration, even in non-targeted areas. This study used HeLa-FUCCI cells to show that while radiation alone suppresses migration, adding cisplatin enhances it, potentially worsening metastasis.

Keywords:
cell migrationchemoradiotherapycisplatinnon-targeted effectradiation-induced bystander effect

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Area of Science:

  • Oncology
  • Cell Biology
  • Radiotherapy Research

Background:

  • Tumor invasion and metastasis significantly impact cancer patient prognosis.
  • Understanding the non-targeted effects of chemoradiotherapy on cell motility is crucial.
  • Cell migration is a key process in tumor invasion and metastasis.

Purpose of the Study:

  • To investigate the impact of chemoradiotherapy on cancer cell migration, including non-targeted effects.
  • To analyze radiation-induced changes in cell motility using a cervical cancer cell line.
  • To determine if chemoradiotherapy enhances or suppresses cell migration in both irradiated and non-irradiated regions.

Main Methods:

  • Utilized HeLa-FUCCI cells for real-time cell cycle phase visualization.
  • Applied a lead block to create distinct irradiated (In-field) and non-irradiated (Out-of-field) zones.
  • Conducted time-lapse imaging for 24 hours post-exposure to 2 Gy X-rays with or without cisplatin.
  • Employed Cellpose 2.0 and TrackMate 7 for cell segmentation and migration tracking analysis.

Main Results:

  • Radiation alone significantly reduced the migration velocity and distance traveled of Out-of-field cells, indicating a bystander suppression effect.
  • In the presence of cisplatin, both In-field and Out-of-field cells exhibited significantly increased migration velocity and total distance traveled.
  • Chemoradiotherapy, specifically with cisplatin, appears to enhance tumor cell motility outside the direct radiation field.

Conclusions:

  • Chemoradiotherapy may have unintended consequences, potentially promoting tumor cell migration and metastasis.
  • The addition of cisplatin to radiotherapy could inadvertently increase the risk of cancer spread.
  • These findings highlight the importance of considering non-targeted effects in cancer treatment strategies to improve patient outcomes.