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Tissue Transplantation

Tissue Transplantation

Tissue transplantation is a significant medical procedure involving the transfer of cells, tissues, or organs from a donor to a recipient, with the primary aim of restoring lost functions. This procedure is crucial in treating a broad spectrum of diseases, including kidney diseases, liver failure, heart disease, and certain types of cancers.
The Biology of Tissue Transplantation
The biology of tissue transplantation hinges on the Major Histocompatibility Complex (MHC) molecules. These molecules...

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Related Experiment Video

Updated: May 8, 2026

Induction of Alloantigen-specific Anergy in Human Peripheral Blood Mononuclear Cells by Alloantigen Stimulation with Co-stimulatory Signal Blockade

Induction of Alloantigen-specific Anergy in Human Peripheral Blood Mononuclear Cells by Alloantigen Stimulation with Co-stimulatory Signal Blockade

Published on: March 14, 2011

Peripheral Nerve Decellularisation Protocol for Allogeneic Transplantation: From Tissue Procurement to Banking.

Marco Govoni¹, Leonardo Vivarelli¹, Nicola Fazio²

¹Reconstructive Orthopaedic Surgery and Innovative Techniques-Musculoskeletal Tissue Bank, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy.

International Journal of Molecular Sciences

|August 28, 2025

Summary

This study developed a decellularisation protocol for human nerves, creating acellular nerve allografts (ANAs) for peripheral nerve repair. These ANAs offer a promising, compliant, and potentially cost-effective alternative to traditional nerve grafts.

Keywords:

ISO Class A cleanroom protocol acellular allogeneic nerve allogeneic nerve transplantation decellularisation

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Last Updated: May 8, 2026

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Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation

Procurement and Decellularization of Rat Hindlimbs Using an Ex Vivo Perfusion-Based Bioreactor for Vascularized Composite Allotransplantation

Published on: June 9, 2022

Area of Science:

Regenerative Medicine
Biomaterials Science
Surgical Innovation

Background:

Peripheral nerve injuries impact over a million people globally each year.
Autologous nerve grafting is standard but has limitations like donor morbidity and tissue scarcity.
Allogeneic nerve grafting presents an alternative but faces immune rejection challenges.

Purpose of the Study:

To adapt and validate a decellularisation protocol for human nerves.
To ensure compliance with European and national regulatory guidelines for tissue banking.
To produce high-quality acellular nerve allografts (ANAs) for peripheral nerve repair.

Main Methods:

Utilised a cleanroom-compliant decellularisation protocol based on mild detergents.
Optimised the protocol to reduce tissue handling time and meet regulatory standards.
Assessed decellularised sural nerves for extracellular matrix preservation and sterility per EU Pharmacopoeia and EDQM guidelines.

Main Results:

Successfully adapted and validated a decellularisation protocol for human nerves.
Demonstrated preservation of extracellular matrix structural and regenerative properties.
Confirmed sterility of the produced acellular nerve allografts (ANAs).

Conclusions:

The developed protocol enables the production of high-quality acellular nerve allografts (ANAs).
These ANAs are suitable for peripheral nerve repair, addressing limitations of current methods.
This research paves the way for accessible and cost-effective nerve grafting solutions.